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This reference work presents the origins of cells for tissue
engineering and regeneration, including primary cells,
tissue-specific stem cells, pluripotent stem cells and
trans-differentiated or reprogrammed cells. There is particular
emphasis on current understanding of tissue regeneration based on
embryology and evolution studies, including mechanisms of amphibian
regeneration. The book covers the use of autologous versus
allogeneic cell sources, as well as various procedures used for
cell isolation and cell pre-conditioning , such as cell sorting,
biochemical and biophysical pre-conditioning, transfection and
aggregation. It also presents cell modulation using growth factors,
molecular factors, epigenetic approaches, changes in biophysical
environment, cellular co-culture and other elements of the cellular
microenvironment. The pathways of cell delivery are discussed with
respect to specific clinical situations, including delivery of ex
vivo manipulated cells via local and systemic routes, as well as
activation and migration of endogenous reservoirs of reparative
cells. The volume concludes with an in-depth discussion of the
tracking of cells in vivo and their various regenerative activities
inside the body, including differentiation, new tissue formation
and actions on other cells by direct cell-to-cell communication and
by secretion of biomolecules.
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