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Protein expression in a heterologous host is a cornerstone of
biomedical research and of the biotechnology industry. Despite the
advanced state of protein expression technology improvements are
still needed. For example, membrane proteins constitute a
significant percentage of the total cellular proteins but as a
class are very difficult to overexpress, especially in a
heterologous host. The ideal host would have the ability to express
any protein, with relevant post-translational modifications, and be
as easy to work with as E. coli. In Heterologous Gene Expression in
E. coli: Methods and Protocols, expert scientists intimately
familiar with the relevant techniques offer chapters that greatly
expand the utility of this expression host. The contributions in
this detailed volume describe methods, for example, to successfully
express proteins in E. coli that would otherwise form aggregates in
this host, to add post-translational modifications, to incorporate
non-standard amino acid residues or moieties into E. coli expressed
proteins, to identify binding partners, and to express membrane
proteins. Written in the highly successful Methods in Molecular
Biology (TM) format, chapters include introductions to their
respective subjects, lists of the necessary materials and reagents,
step-by-step, readily reproducible laboratory protocols, and tips
on troubleshooting and avoiding known pitfalls. Practical and
cutting-edge, Heterologous Gene Expression in E. coli: Methods and
Protocols seeks to familiarize the researcher with the myriad of E.
coli expression strains available and move E. coli closer to that
ideal of the perfect host.
Protein expression in a heterologous host is a cornerstone of
biomedical research and of the biotechnology industry. Despite the
advanced state of protein expression technology improvements are
still needed. For example, membrane proteins constitute a
significant percentage of the total cellular proteins but as a
class are very difficult to overexpress, especially in a
heterologous host. The ideal host would have the ability to express
any protein, with relevant post-translational modifications, and be
as easy to work with as E. coli. In Heterologous Gene Expression in
E. coli: Methods and Protocols, expert scientists intimately
familiar with the relevant techniques offer chapters that greatly
expand the utility of this expression host. The contributions in
this detailed volume describe methods, for example, to successfully
express proteins in E. coli that would otherwise form aggregates in
this host, to add post-translational modifications, to incorporate
non-standard amino acid residues or moieties into E. coli expressed
proteins, to identify binding partners, and to express membrane
proteins. Written in the highly successful Methods in Molecular
Biology(TM) format, chapters include introductions to their
respective subjects, lists of the necessary materials and reagents,
step-by-step, readily reproducible laboratory protocols, and tips
on troubleshooting and avoiding known pitfalls. Practical and
cutting-edge, Heterologous Gene Expression in E. coli: Methods and
Protocols seeks to familiarize the researcher with the myriad of E.
coli expression strains available and move E. coli closer to that
ideal of the perfect host.
Title: History of Llangynwyd parish, with illustrations.Publisher:
British Library, Historical Print EditionsThe British Library is
the national library of the United Kingdom. It is one of the
world's largest research libraries holding over 150 million items
in all known languages and formats: books, journals, newspapers,
sound recordings, patents, maps, stamps, prints and much more. Its
collections include around 14 million books, along with substantial
additional collections of manuscripts and historical items dating
back as far as 300 BC.The HISTORY OF BRITAIN & IRELAND
collection includes books from the British Library digitised by
Microsoft. As well as historical works, this collection includes
geographies, travelogues, and titles covering periods of
competition and cooperation among the people of Great Britain and
Ireland. Works also explore the countries' relations with France,
Germany, the Low Countries, Denmark, and Scandinavia. ++++The below
data was compiled from various identification fields in the
bibliographic record of this title. This data is provided as an
additional tool in helping to insure edition identification: ++++
British Library Evans, Thomas C.; 1887. vii. 192 p.; 8 .
10369.dd.3.
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