This second volume provides new and updated methods detailing advancements in CRISPR-Cas technical protocols. Chapters guide readers through protocols on prime editing, base editing, multiplex editing, editing in cell-free extract, in silico analysis of gRNA secondary structure and CRISPR-diagnosis. Authoritative and cutting-edge, CRISPR-Cas Methods, Volume 2 aims to serves as a laboratory manual providing scientists with a holistic view of CRISPR-Cas methodologies and its practical application for the editing of crop plants, cell lines, nematode and microorganism. The chapter "CRISPR/Cas9-mediated gene editing in human induced pluripotent stem cells" is available open access under a Creative Commons Attribution 4.0 International License via link.springer.com.

Agricultural biostimulants are a group of substances or microorganisms, based on natural resources, that are applied to plants or soils to improve nutrient uptake and plant growth, and provide better tolerance to various stresses. Their function is to stimulate the natural processes of plants, or to enrich the soil microbiome to improve plant growth, nutrition, abiotic and/or biotic stress tolerance, yield and quality of crop plants. Interest in plant biostimulants has been on the rise over the past 10 years, driven by the growing interest of researchers and farmers in environmentally-friendly tools for improved crop performance. Focusing on recent progress on biostimulants and their role in crop production and agricultural sustainability, this book includes: 31 chapters on a wide range of biostimulants and their role in plant growth stimulation and stress tolerance. Mechanism of actions of diverse groups of biostimulants, such as trace elements, plant and seaweed extracts, humic substances, polyamines, osmolytes, vitamins, nanoparticles and microorganisms. New promising biostimulants with novel modes of action. Improved crop production technologies are urgently needed to meet the growing demand for food for the ever-increasing global population by addressing the impacts of changing climate on agriculture. This book is of interest to researchers in agriculture, agronomy, crop and plant science, soil science and environmental science.

Global climate change affects crop production through altered weather patterns and increased environmental stresses. Such stresses include soil salinity, drought, flooding, metal/metalloid toxicity, pollution, and extreme temperatures. The variability of these environmental conditions pared with the sessile lifestyle of plants contribute to high exposure to these stress factors. Increasing tolerance of crop plants to abiotic stresses is needed to fulfill increased food needs of the population. This book focuses on methods of improving plants tolerance to abiotic stresses. It provides information on how protective agents, including exogenous phytoprotectants, can mitigate abiotic stressors affecting plants. The application of various phytoprotectants has become one of the most effective approaches in enhancing the tolerance of plants to these stresses. Phytoprotectants are discussed in detail including information on osmoprotectants, antioxidants, phytohormones, nitric oxide, polyamines, amino acids, and nutrient elements of plants. Providing a valuable resource of information on phytoprotectants, this book is useful in diverse areas of life sciences including agronomy, plant physiology, cell biology, environmental sciences, and biotechnology.

Plants are frequently exposed to unfavorable and adverse environmental conditions known as abiotic stressors. These factors can include salinity, drought, heat, cold, flooding, heavy metals, and UV radiation which pose serious threats to the sustainability of crop yields. Since abiotic stresses are major constraints for crop production, finding the approaches to enhance stress tolerance is crucial to increase crop production and increase food security. This book discusses approaches to enhance abiotic stress tolerance in crop plants on a global scale. Plants scientists and breeders will learn how to further mitigate plant responses and develop new crop varieties for the changing climate.

Plants are frequently exposed to unfavorable and adverse environmental conditions known as abiotic stressors. These factors can include salinity, drought, heat, cold, flooding, heavy metals, and UV radiation which pose serious threats to the sustainability of crop yields. Since abiotic stresses are major constraints for crop production, finding the approaches to enhance stress tolerance is crucial to increase crop production and increase food security. This book discusses approaches to enhance abiotic stress tolerance in crop plants on a global scale. Plants scientists and breeders will learn how to further mitigate plant responses and develop new crop varieties for the changing climate.

Global climate change affects crop production through altered weather patterns and increased environmental stresses. Such stresses include soil salinity, drought, flooding, metal/metalloid toxicity, pollution, and extreme temperatures. The variability of these environmental conditions pared with the sessile lifestyle of plants contribute to high exposure to these stress factors. Increasing tolerance of crop plants to abiotic stresses is needed to fulfill increased food needs of the population. This book focuses on methods of improving plants tolerance to abiotic stresses. It provides information on how protective agents, including exogenous phytoprotectants, can mitigate abiotic stressors affecting plants. The application of various phytoprotectants has become one of the most effective approaches in enhancing the tolerance of plants to these stresses. Phytoprotectants are discussed in detail including information on osmoprotectants, antioxidants, phytohormones, nitric oxide, polyamines, amino acids, and nutrient elements of plants. Providing a valuable resource of information on phytoprotectants, this book is useful in diverse areas of life sciences including agronomy, plant physiology, cell biology, environmental sciences, and biotechnology.

The third volume of the series ‘Bacilli and Agrobiotechnology’ is comprised of 25 chapters that bring a unique perspective to the readers about Bacillus-mediated biotic and abiotic plant stress tolerance, bioremediation and bioprospecting. These chapters are prepared by the leading scientists of global repute. The negative impacts of agrochemicals such as chemical fertilizers and pesticides on human health and environment are paramount. Bacillus and allied genera of beneficial plant-associated microbes are presenting beacon of hope to the farmers, plant scientists and stewards of environment. Several chapters of this volume focus on the induction of various signaling pathways in plants by Bacillus spp. to alleviate biotic and abiotic stresses impacted by global climate change Agricultural lands contaminated with heavy metals affect the ecological food chain starting from crop cultivation. How the toxic effects of trace metals originating from industrial effluents and agrochemicals can be remediated? This book addresses how to overcome these issues by applying elite strains of Bacillus. Bioprospecting is a systematic and organized search for conversion of bioresources to industrially important products by utilizing microbe-derived metabolites. This volume is enriched by including the bioprospecting aspects mediated by Bacillus spp. with novel insights.

Crop plants growing under field conditions are constantly exposed to various abiotic and biotic stress factors leading to decreased yield and quality of produce. In order to achieve sustainable development in agriculture and to increase agricultural production for feeding an increasing global population, it is necessary to use ecologically compatible and environmentally friendly strategies to decrease the adverse effects of stresses on the plant. Selenium is one of the critical elements from the biological contexts because it is essential for human health; however, it becomes toxic at high concentrations. It has been widely reported that selenium can promote plant growth and alleviate various stresses as well as increase the quantity and quality of the yield of many plant species. Nonetheless, at high concentrations, selenium causes phytotoxicity. In the last decade, nanotechnology has emerged as a prominent tool for enhancing agricultural productivity. The production and applications of nanoparticles (NPs) have greatly increased in many industries, such as energy production, healthcare, agriculture, and environmental protection. The application of NPs has attracted interest for their potential to alleviate abiotic and biotic stresses in a more rapid, cost-effective, and more sustainable way than conventional treatment technologies. Recently, research related to selenium-NPs-mediated abiotic stresses and nutritional improvements in plants has received considerable interest by the scientific community. While significant progress was made in selenium biochemistry in relation to stress tolerance, an in-depth understanding of the molecular mechanisms associated with the selenium- and nano-selenium-mediated stress tolerance and bio-fortification in plants is still lacking. Gaining a better knowledge of the regulatory and molecular mechanisms that control selenium uptake, assimilation, and tolerance in plants is therefore vital and necessary to develop modern crop varieties that are more resilient to environmental stress. This book provides a comprehensive overview of the latest understanding of the physiological, biochemical, and molecular basis of selenium- and nano-selenium-mediated environmental stress tolerance and crop quality improvements in plants. It helps researchers to develop strategies to enhance crop productivity under stressful conditions and to better utilize natural resources to ensure future food security and to reduce environmental contamination. Finally, this book is a valuable resource for promoting future research into plant stress tolerance, and a reference book for researchers working on developing plants tolerant to abiotic and biotic stressors as well as bio-fortification and phytoremediation.

This second volume provides new and updated methods detailing advancements in CRISPR-Cas technical protocols. Chapters guide readers through protocols on prime editing, base editing, multiplex editing, editing in cell-free extract, in silico analysis of gRNA secondary structure and CRISPR-diagnosis. Authoritative and cutting-edge, CRISPR-Cas Methods, Volume 2 aims to serves as a laboratory manual providing scientists with a holistic view of CRISPR-Cas methodologies and its practical application for the editing of crop plants, cell lines, nematode and microorganism. The chapter "CRISPR/Cas9-mediated gene editing in human induced pluripotent stem cells" is available open access under a Creative Commons Attribution 4.0 International License via link.springer.com.

The Gram-positive and spore-forming Bacilli are the most dominant group of bacteria that exist in various ecological niches on the earth. They represent one of the most important unmapped pools of biodiversity with immense potential of applications in agriculture, environment, and industry. As these bacteria are highly tolerant to stressful environment and enhance plant tolerance to harsh environment such as salinity, drought, and heavy metal toxicity, plant-associated Bacilli have high potential for promoting sustainable crop production. Many species of Bacilli are being commercially used as phytostimulator and biofertilizer. Some of them are applied as biopesticide for protecting crop plants from phytopathogens and insect pests. The Bacillus-based products are becoming popular in ecologically sound and climate resilient agricultural production system. In fact, Bacillus and allied species based formulations are already dominating the biopesticides market, although, to compete with other formulations and chemical alternatives, the biology of Bacillus had to be understood from perspective of such applications. Our understanding of the biology and molecular-basis of the beneficial effects of plant-associated Bacilli has greatly been progressed in recent years through genomics, metagenomics, post-genomics and metabolomics studies. The volume two of the series Bacilli and Agrobiotechnology comprehensively reviews and updates current knowledge of Bacilli as phytostimulant and biological control of plant pests. Better understanding the biology, ecology and mechanism of action of the beneficial strains of Bacilli will play a role in the development of products to support green biotechnology in agriculture and industries.

Feeding of powdered black seeds of historically famous medicinal herb, Nigella sativa L. has been shown to lower serum triglycerides and decrease egg cholesterol in laying hens. This study aimed to investigate effects of varying doses of N. sativa seed powder (0, 1.5, 2.5 and 3.0%) and acetone extracts (0, 1.5 and 3.0%) on feed intake, health, fat content, serum lipid profiles and population of intestinal microflora of broiler. Results revealed that N. sativa supplemented feed had no significant effects on feed intake, body weight, fat content and mortality rate. However, supplementation of 3.0% seed powder and 0.4% extract of N. sativa significantly (p

Phosphate predominantly present as inorganic compounds, which are either calcium or iron and aluminum salts. Iron and aluminum phosphates predominate under acidic condition. The low availability of P to plants is because the vast majority of soil P is found in insoluble forms .Thus, the release of insoluble and fixed forms of phosphorus is an important aspect of increasing soil phosphorus availability.Plant root-associated phosphate solubilizing bacteria (PSB) have been considered as one of the possible alternatives for inorganic phosphate fertilizers for promoting plant growth and yield. Considering the above stated role of bacteria for P solubilization in acid soils of Bangladesh, the present work was therefore undertaken with the following objectives: To isolate some rhizoplane bacteria from the roots of rice plants and screening potential phosphate solubilizing bacteria (PBS) from the isolates; To quantify P solubilization from tricalcium phosphate by potential PSB in broth culture conditions; and To see the performances of potential PSB on growth and nutrients content of rice in acidic soil of Bangladesh.