This second volume provides new and updated methods detailing
advancements in CRISPR-Cas technical protocols. Chapters guide
readers through protocols on prime editing, base editing, multiplex
editing, editing in cell-free extract, in silico analysis of gRNA
secondary structure and CRISPR-diagnosis. Authoritative and
cutting-edge, CRISPR-Cas Methods, Volume 2 aims to serves as a
laboratory manual providing scientists with a holistic view of
CRISPR-Cas methodologies and its practical application for the
editing of crop plants, cell lines, nematode and microorganism. The
chapter "CRISPR/Cas9-mediated gene editing in human induced
pluripotent stem cells" is available open access under a Creative
Commons Attribution 4.0 International License via
link.springer.com.
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