Diverse molecular, cellular, and environmental events must all come together to allow the successful formation of secondary cancers, metastases. The second edition of Metastasis Research Protocols, brings together the most up to date versions of the seminal techniques that were presented in the first edition and also includes new techniques that have recently been shown to be important in illuminating the processes underlying this important area of biology. Presented by top scientists, the collection includes a wide spectrum of articles encompassing important key methods and to introduce new methods which are making an impact in the area of metastasis research. Volume 1 includes key cellular and molecular techniques relevant to the exploration of cancer cells and tissues, the focus is on the tools that have been shown to be helpful in unravelling the molecular processes important in cancer metastasis. Written in the highly successful Methods in Molecular Biology (TM) series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Metastasis Research Protocols, Second Edition seeks to aid scientists in the further study of new methods in the area of metastasis research.

Diverse molecular, cellular, and environmental events must all come together to allow the successful formation of secondary cancers, metastases. The second edition of Metastasis Research Protocols, brings together updated versions of the seminal technique that were presented in the first edition and also includes new techniques that have recently been shown to be important in illuminating the processes underlying this important area of biology. Volume 2 presents techniques applicable at the level of living cells and tissues, and presents methodologies applicable to cell behaviour in vitro, in animal models and in mathematical constructs. The aim is the study of the interaction between cancer cells and their host/environment. The focus throughout is on the tools that have been shown to be helpful in unravelling the processes important in cancer metastasis. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Metastasis Research Protocols, Second Edition seeks to aid scientists in the further study of new methods in the area of metastasis research.

Quintessential Atlas of Anatomy expands on widely acclaimed prior editions! Atlas of Anatomy, Latin Nomenclature, Fourth Edition builds on its longstanding reputation of being the highest-quality anatomy atlas published to date using Latin nomenclature. With more than 2,000 exquisitely detailed illustrations, including over 120 new to this edition, the Atlas helps students and seasoned clinicians master the details of human anatomy. Key Features: NEW! Expanded Radiology sections include over 40 new radiographs, CTs, and MRIs NEW! A more dissectional approach to the head and neck region places neck anatomy before that of the head – the way most students dissect NEW! Additional images and tables detail the challenging anatomy of the peritoneal cavity, inguinal region, and infratemporal and pterygopalatine fossae NEW! Almost 30 new clinical boxes focus on function, pathology, diagnostic techniques, anatomic variation, and more NEW! More comprehensive coverage clarifies the complexities of the ANS, including revised wiring schematics Also included in this new edition: Muscle Fact spreads provide origin, insertion, innervation, and action An innovative, user-friendly format: every topic covered in two side-by-side pages Online images with "labels-on and labels-off" capability are ideal for review and self-testing What users say about the Atlas of Anatomy: "I can't say enough how much I like the organization of this text. I think Thieme has 'hit the nail on the head' with structuring everything by region (Lower Limb) and sub-region (Ankle & Foot). It's very easy to find what you're looking for…" "The figures in the Atlas of Anatomy are exemplary and surpass other competing texts. The images are clear, precise, and aesthetically colored. The unique views presented in this work are also very helpful for studying a three-dimensional subject such as human anatomy." This book includes complimentary access to a digital copy on https://medone.thieme.com.

Remarkable atlas provides exceptionally detailed, clinically relevant anatomic knowledge! Thieme Atlas of Anatomy: General Anatomy and Musculoskeletal System, Third Edition, Latin Nomenclature, by renowned educators Michael Schuenke, Erik Schulte, and Udo Schumacher, along with consulting editors Nathan Johnson and Hugo Zeberg, expands on the award-winning prior editions with updated spreads and added information on joints, muscle actions, and functional muscle groups. Organized by region, the book begins with an introduction on basic human embryology and development and an overview of the human body. Subsequent general anatomy chapters explore surface anatomy, the bones, joints, muscles, vessels, lymphatic system and glands, and general neuroanatomy. The next section delineates the trunk wall, functional musculature, and the neurovascular system, while the last two sections are dedicated to the upper limb and lower limb. Key Features Labels and anatomic terminology are in Latin nomenclature Nearly 2,100 images including extraordinarily realistic illustrations by Markus Voll and Karl Wesker, X-rays, MRIs, CT scans, diagrams, tables, and descriptive text provide an unparalleled wealth of information about muscle structure and bones Musculoskeletal, vascular, and nervous system structures are presented systematically first, then topographically, thereby supporting classroom learning and active laboratory dissection Emphasizes important relationships between anatomic structure and function in addition to introducing clinical applications, providing knowledge trainees can apply in practice Online images with "labels-on and labels-off" capability are ideal for review and self-testing This visually stunning atlas is a must have for medical, allied health, and physical therapy students, instructors, and practicing physical and massage therapists. It is also a wonderful anatomic reference for professional artists and illustrators. The THIEME Atlas of Anatomy series also includes two additional volumes, Internal Organs and Head, Neck, and Neuroanatomy. All volumes of the THIEME Atlas of Anatomy series are available in softcover English/International nomenclature and in hardcover with Latin nomenclature. This book includes complimentary access to a digital copy on https://medone.thieme.com.

Remarkable atlas provides exceptionally detailed, clinically relevant anatomic knowledge! Thieme Atlas of Anatomy: Head, Neck, and Neuroanatomy, Third Edition, Latin Nomenclature, by renowned educators Michael Schuenke, Erik Schulte, and Udo Schumacher, along with consulting editors Cristian Stefan and Hugo Zeberg, expands on prior editions with hundreds of new images and significant updates to the neuroanatomy content. Head and neck sections encompass the bones, ligaments, joints, muscles, lymphatic system, organs, related neurovascular structures, and topographical and sectional anatomy. The neuroanatomy section covers the histology of nerve and glial cells and autonomic nervous system, then delineates different areas of the brain and spinal cord, followed by sectional anatomy and functional systems. The final section features a glossary and expanded CNS synopses, featuring six new topics, from neurovascular structures of the nose to the pharynx. Key Features Labels and anatomic terminology are in Latin nomenclature Nearly 1,800 images including extraordinarily realistic illustrations by Markus Voll and Karl Wesker, photographs, diagrams, tables, and succinct clinical applications make this the perfect study and teaching resource Expanded clinical references include illustrated summary tables and synopses of motor and sensory pathways Neuroanatomy additions include an in-depth overview and content focused on functional circuitry and pathways Online images with "labels-on and labels-off" capability are ideal for review and self-testing This visually stunning atlas is an essential companion for medical students or residents interested in pursuing head and neck subspecialties or furthering their knowledge of neuroanatomy. It will also benefit dental and physical therapy students, as well as physicians and physical therapists seeking an image-rich clinical resource to consult in practice. The THIEME Atlas of Anatomy series also includes two additional volumes, General Anatomy and Musculoskeletal System and Internal Organs. All volumes of the THIEME Atlas of Anatomy series are available in softcover English/International nomenclature and in hardcover with Latin nomenclature. This book includes complimentary access to a digital copy on https://medone.thieme.com.

Diverse molecular, cellular, and environmental events must all come together to allow the successful formation of secondary cancers, metastases. The second edition of Metastasis Research Protocols, brings together updated versions of the seminal technique that were presented in the first edition and also includes new techniques that have recently been shown to be important in illuminating the processes underlying this important area of biology. Volume 2 presents techniques applicable at the level of living cells and tissues, and presents methodologies applicable to cell behaviour in vitro, in animal models and in mathematical constructs. The aim is the study of the interaction between cancer cells and their host/environment. The focus throughout is on the tools that have been shown to be helpful in unravelling the processes important in cancer metastasis. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Metastasis Research Protocols, Second Edition seeks to aid scientists in the further study of new methods in the area of metastasis research.

In Volume I, Analysis of Cells and Tissues, we presented a range of protocols aimed at mapping and analyzing the expression of various molecules of pot- tial interest in metastasis research and for examining their production at the genetic level. In this second volume of metastasis research protocols, we move to the level of living cells and tissues and present methodologies applicable to examining metastatic behavior in vitro and in whole animal models. The methods described in the first section of this volume concentrate on the separation of cell lines with high and low metastatic potential, including the genetic modification of cell lines. The assay systems to test defined aspects of the metastatic cascade are then described in Part II and include cell migration assays, assays for matrix degrading enzymes, basement membrane degrading assays, adhesion assays, and assays of angiogenesis. The role of the specific elements of the metastatic cascade assayed in each of these systems in turn must of course be put into perspective relative to their roles in entire living organisms.

The process of metastasis formation is hugely complex, as described in the introductory chapter of this book, and this complexity has led us to compile two volumes of methods, from a vastly divergent background that attempts to encompass the whole spectrum of cancer biology. This first volume, Metastasis Research Protocols: Analysis of Cells and Tissues, concentrates on analysis and mapping of molecules produced by cells and tissues and analysis of the molecular biology underlying their expression, whereas the second volume, Metastasis Research Protocols: Cell Behavior In Vitro and In Vivo,focuses sharply on the determination of cell behavior in vitro and in vivo. We have deliberately included chapters describing well-established and familiar te- niques (for example, SDS-PAGE and Western blotting [Chapter 11], and immunocytochemistry [Chapter 2]) in addition to the newer and more speci- ized approaches and specific examples of their application, because-although the methodology is readily available in the published literature and established in many laboratories-we wished these volumes to "stand alone" and to make accessible here the standard techniques that underpin much metastasis research for both the newcomer to the field and the seasoned researcher. Undoubtedly, owing to the complexity of the metastatic cascade and the wealth of research techniques involved in scientific approaches to its unraveling, and despite our best efforts to make these volumes as comprehensive as seems feasible, this is a tall order, and there will inevitably be omissions. For these we apologize.

Remarkable atlas provides exceptionally detailed, clinically relevant anatomic knowledge! Thieme Atlas of Anatomy: Internal Organs, Third Edition, Latin Nomenclature, by renowned educators Michael Schuenke, Erik Schulte, Udo Schumacher, along with consulting editors Wayne Cass and Hugo Zeberg, expands on prior editions with increased detail on anatomic relationships of inner organs, and the innervation and lymphatic systems of these organs. Organized by region, the book features 10 sections starting with an overview on body cavities. Subsequent sections cover the cardiovascular, blood, lymphatic, respiratory, digestive, urinary, genital, endocrine, and autonomic nervous organ systems. Regional units covering the thorax and abdomen and pelvis begin with succinct overviews, followed by more in-depth chapters detailing the structure and neurovasculature of the region and its organs. Key Features Labels and anatomic terminology are in Latin nomenclature 1,375 images including extraordinarily realistic illustrations by Markus Voll and Karl Wesker, diagrams, tables, and descriptive text provide an unparalleled wealth of information about internal organs 21 fact sheets provide quick, handy references summarizing salient points for each organ Online images with "labels-on and labels-off" capability are ideal for review and self-testing This visually stunning atlas is an essential companion for laboratory dissection and the classroom. It will benefit medical students, internal medicine residents, and practicing physicians. The THIEME Atlas of Anatomy series also includes two additional volumes, General Anatomy and Musculoskeletal System and Head, Neck, and Neuroanatomy. All volumes of the THIEME Atlas of Anatomy series are available in softcover English/International nomenclature and in hardcover with Latin nomenclature. This book includes complimentary access to a digital copy on https://medone.thieme.com.

Diverse molecular, cellular, and environmental events must all come together to allow the successful formation of secondary cancers, metastases. The second edition of Metastasis Research Protocols, brings together the most up to date versions of the seminal techniques that were presented in the first edition and also includes new techniques that have recently been shown to be important in illuminating the processes underlying this important area of biology. Presented by top scientists, the collection includes a wide spectrum of articles encompassing important key methods and to introduce new methods which are making an impact in the area of metastasis research. Volume 1 includes key cellular and molecular techniques relevant to the exploration of cancer cells and tissues, the focus is on the tools that have been shown to be helpful in unravelling the molecular processes important in cancer metastasis. Written in the highly successful Methods in Molecular Biology (TM) series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Metastasis Research Protocols, Second Edition seeks to aid scientists in the further study of new methods in the area of metastasis research.

A visually-rich and clinically relevant anatomy resource for dental students Anatomy for Dental Medicine, Third Edition strikes an optimal balance between systemic and regional approaches to complex head and neck anatomy. Award-winning full-color illustrations, succinct text, summary tables, and questions put anatomical structures and knowledge into a practical context. Key Features NEW! Additional radiologic images and landmark features throughout NEW! Reorganized brain/nerve sections NEW! Expanded clinical question appendix including patient box questions in the style of the INBDE NEW! Factual question appendix places greater emphasis on areas including the skull, larynx, cross sectional anatomy, body below the neck, and local anesthesia Also included in this new edition: 1,200 clear and detailed full-color illustrations 150 tables for rapid access to key information Expanded captions detail key information and clinical correlations Appendix covering anatomy for local anesthesia Online images with "labels-on and labels-off" capability are ideal for review and self-testing This is an essential resource for dental students and residents that also provides a robust review for the boards or dissection courses. What users say about the Atlas of Anatomy: "…I am impressed with the 2nd edition of the atlas because it provides multiple, complimentary paths to learning head and neck anatomy." "…organization is in an effective sequence that walks the learner through the anatomy in a layered, step-wise manner." This book includes complimentary access to a digital copy on https://medone.thieme.com.

Anatomy for Dental Medicine, Latin Nomenclature, combines award-winning, full-color illustrations, explanatory text, and summary tables to guide the reader through the complex anatomy of the head and neck as well as other body regions relevant to dental medicine. Each region is arranged in a user-friendly format beginning with the skeletal framework. The musculature is then added, followed by the neurovasculature, and finally, topographic anatomy shows all structures in situ. Key features: All labels and anatomic terminology are in Latin nomenclature Over 1,200 clear, detailed, full-color illustrations Expanded captions elucidate key concepts and contain relevant clinical correlations Over 150 tables for quick access to key information Includes embryology and rest of body anatomy Expanded neuroanatomy chapter Sectional anatomy chapter that includes radiographic images to facilitate clinical understanding Appendix covering the anatomy for local anesthesia with step-by-step instructions for these important procedures Appendices with review questions and answers, both factual and clinical-vignette style Anatomy for Dental Medicine, Latin Nomenclature, includes access to WinkingSkull.com PLUS, the interactive online study aid, with all full-color illustrations and radiographs from this volume and the review questions and answers in an interactive format. Review or test your anatomy knowledge with timed self-tests using the labels on-and-off function on the illustrations, with access to instant results.