The primary purpose of this volume is to demonstrate the range of applications of the Pyrosequencing technology in research and diagnostics and to provide detailed protocols. Beginning with an up-to-date overview of the biochemistry, the volume continues with quantitative analysis of genetic variation, ratio of expressed alleles at the RNA level, analysis of DNA methylation, global DNA methylation assays, specialized applications for DNA methylation analysis including loss of imprinting, single blastocyst analysis, allele-specific DNA methylation patterns, DNA methylation patterns associated with specific histone modifications. The volume further details tools and protocols for the detection of viruses and bacteria, and genetic and epigenetic analyses for forensics using Pyrosequencing. As a volume in the highly successful Methods in Molecular Biology series, chapters contain introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols and tips on troubleshooting and avoiding known pitfalls. Comprehensive and adaptable, Pyrosequencing: Methods and Protocols, Second Edition will greatly aid doctorial students, postdoctoral investigators and research scientists studying different aspects of genetics and cellular and molecular biology.

A landmark survey of the work of Isaac Mizrahi, a trailblazing and influential American fashion designer, artist, and entrepreneur Beginning with Isaac Mizrahi's first fashion collection, which debuted to critical acclaim in 1986, and running though the present day, this stylish, lavishly illustrated book presents his signature couture collections. Mizrahi's exuberant couture style is classic American, inventively reimagined. He pioneered the concept of "high/low" in fashion, and was the first high-end fashion designer to create an accessibly priced mass-market line. Mizrahi approached other complex issues through his designs, as well-mixing questions of beauty and taste with those of race, religion, class, and politics. Although Mizrahi (b. 1961) is best known for his clothing, his work in theater, film, and television is also explored. The result is a spirited discourse on high versus low, modern glamour, and contemporary culture. Three essayists discuss Mizrahi's place in fashion history, his close connection to contemporary art, and the performative nature of his designs. New photography brings Mizrahi's fashions to life, and an interview with the artist offers an intimate perspective on his kaleidoscopic work in diverse media. Published in association with the Jewish Museum, New York Exhibition Schedule: Jewish Museum, New York (03/18/16-08/07/16)

A cultural and historical philosophy of fashion in economic and social life from the 1830s to the present dayUlrich Lehmann brings together methods and ideas from social sciences and material production to offer a new political reading of fashion in today's post-democracy. Accessing rare source material across a wide range of European languages and cultures, he offers insight into new working structures in the manufacture of garments and textiles. Reinvigorates materialism as a critical approach to analysing economics, society and media through the thematic focus on fashion as the economically and culturally dominant sector within post-industrial societiesCase studies include the male suit in Alfred Hitchcock's film 'North by Northwest' (1959), the revolutionary production methods in the work of Carol Christian Poell and the innovative textile manufacture of Bonotto in Molvena, north-east ItalyRedirects fashion theory toward materiality and materialism from previous art-historical and social-anthropological approachesExposes the need critically to engage with fashion production, away from the exclusive reading of fashion through its media representationExtends the discussion of fashion production from aspects of labour conditions and sustainability to the materialist critique of the fashion system

Dieser unveranderte Nachdruck der 4. Auflage von 1996 macht das nachgefragte Woerterbuch wieder verfugbar. Das Buch erklart die Fachausdrucke der Palaontologie verstandlich und ermoeglicht somit den Zugang zum Eigentlichen dieser Wissenschaft. Es ist fur alle an Palaontologie im weitesten Sinne Interessierte gedacht: Studierende der Geowissenschaften, Fachleute im engeren Sinn, Lehrer, Schuler, besonders auch die zahlreichen Fossilien-Sammler. Die in der Literatur gebrauchlichen Fachausdrucke werden in der moeglichen Kurze erlautert und sprachlich abgeleitet. Sachlich Zusammengehoeriges ist vielfach in eigenen Abschnitten gemeinsam dargestellt; zahlreiche Verweise auf Zusammenhange oder verwandte Begriffe erleichtern die UEbersicht.

Ulrich Lehmann brings together methods and ideas from social sciences and material production to offer a new political reading of fashion in today's post-democracy. Accessing rare source material across a wide range of European languages and cultures, he offers insight into new working structures in the manufacture of garments and textiles. Case studies include the male suit in Alfred Hitchcock's film North by Northwest (1959), the revolutionary production methods in the work of Carol Christian Poell and the innovative textile manufacture of Bonotto in Molvena (north-East Italy)