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The efficiency of delivering DNA into mammalian cells has increased
t- mendously since DEAE dextran was first shown to be capable of
enhancing transfer of RNA into mammalian cells in culture. Not only
have other chemical methods been developed and refined, but also
very efficient physical and viral delivery methods have been
established. The technique of introducing DNA into cells has
developed from transfecting tissue culture cells to delivering DNA
to specific cell types and organs in vivo. Moreover, two important
areas of biology-assessment of gene function and gene
therapy-require succe- ful DNA delivery to cells, driving the
practical need to increase the efficiency and efficacy of gene
transfer both in vitro and in vivo. TM These two volumes of the
Methods in Molecular Biology series, Gene Del- ery to Mammalian
Cells, are designed as a compendium of those techniques that have
proven most useful in the expanding field of gene transfer in
mammalian cells. It is intended that these volumes will provide a
thorough background on chemical, physical, and viral methods of
gene delivery, a synopsis of the myriad techniques currently
available to introduce genes into mammalian cells, as well as a
practical guide on how to accomplish this. It is my expectation
that it will be useful to the novice in the field as well as to the
scientist with expertise in gene delivery.
Highly experienced researchers describe in step-by-step detail
methods that have proven most useful in delivering genes to
mammalian cells. Volume 1: Nonviral Gene Transfer Techniques
focuses on gene delivery by a variety of chemical and physical
methods, including ultrasound, biolistics, peptides, PNA clamps,
liposomes, microinjection, electroporation, particle bombardment,
dendrimers, and hydrodynamics. An accompanying volume, Volume 2:
Viral Gene Transfer Techniques, details procedures for delivering
genes to cells in vitro and in vivo, including the use of
lentiviral vectors.
The efficiency of delivering DNA into mammalian cells has increased
t- mendously since DEAE dextran was first shown to be capable of
enhancing transfer of RNA into mammalian cells in culture. Not only
have other chemical methods been developed and refined, but also
very efficient physical and viral delivery methods have been
established. The technique of introducing DNA into cells has
developed from transfecting tissue culture cells to delivering DNA
to specific cell types and organs in vivo. Moreover, two important
areas of biology-assessment of gene function and gene
therapy-require succe- ful DNA delivery to cells, driving the
practical need to increase the efficiency and efficacy of gene
transfer both in vitro and in vivo. TM These two volumes of the
Methods in Molecular Biology series, Gene Del- ery to Mammalian
Cells, are designed as a compendium of those techniques that have
proven most useful in the expanding field of gene transfer in
mammalian cells. It is intended that these volumes will provide a
thorough background on chemical, physical, and viral methods of
gene delivery, a synopsis of the myriad techniques currently
available to introduce genes into mammalian cells, as well as a
practical guide on how to accomplish this. It is my expectation
that it will be useful to the novice in the field as well as to the
scientist with expertise in gene delivery.
Highly experienced researchers describe in step-by-step detail
methods that have proven most useful in delivering genes to
mammalian cells. Volume 1: Nonviral Gene Transfer Techniques
focuses on gene delivery by a variety of chemical and physical
methods, including ultrasound, biolistics, peptides, PNA clamps,
liposomes, microinjection, electroporation, particle bombardment,
dendrimers, and hydrodynamics. An accompanying volume, Volume 2:
Viral Gene Transfer Techniques, details procedures for delivering
genes to cells in vitro and in vivo, including the use of
lentiviral vectors.
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