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This volume discusses different approaches towards understanding
the brain plasticity, aging, and regeneration of the nervous system
in model organisms. Chapters guide the reader through an overview
of model organisms, setup of an aquatic system, Tol2-mediated
transgenesis, CRISPR/Cas9-mediated gene knock-out and knock-in,
Cas13d-mediated gene knockdown, tissue clearing and expansion
methods for brain and spinal cord imaging, Histological techniques,
chromatin Immunoprecipitation Sequencing (ChIP-Seq) analysis,
single-cell RNA sequencing analysis, BrdU/EdU incorporation assays,
brain and spinal cord injury in African killifish, and emerging
technologies and future perspectives in African killifish research.
In the Neuromethods series style, chapters include the kind of
detail and key advice from the specialists needed to get successful
results in your laboratory. Authoritative and cutting-edge,
Emerging Model Organisms aims to be a useful practical guide to
researches to help further their study in this field.
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