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The book provides a general introduction to advanced computational
techniques applied in various stages of drug discovery. In
particular, it focuses on molecular modeling, ligand- and
structure-based drug design methods, and data mining in chemical
informatics. Application side of this book deals with the
development of the first structure-based three-dimensional
quantitative structure-activity relationship (3D-QSAR) model of
butyrylcholinesterase - a putative drug target for the treatment of
Alzheimer's disease. The other part is comprised of detailed work
conducted on 5-HT2C agonists. It describes pharmacophore modeling
and 3D-QSAR studies. Comparative Molecular Field Analysis coupled
with a genetic algorithm (CoMFA-GA) was carried out for aligned
ligands. The last part describes the drug target MTB-Thymidine
monophosphate kinase (TMPKMtub). The molecular modeling analyses
resulted in identification of a cluster of water molecules that
mediate key interactions between bound ligands and backbone atoms
of the enzyme residues.
Structure Prediction is one of the most important aspects of
Structural Bioinformatics, Protein Docking and Drug Designing. The
current study is performed on MTHFR enzyme. It plays an important
role in folate and homocysteine metabolism by catalyzing the
conversion of 5,10-methylenetetrahydrofolate to 5-
methyltetrahydrofolate, used for homocysteine remethylation to
methionine. MTHFR mutations have been found in a large number of
diseases. However, the structure of MTHFR is still unknown, thereby
limiting the understanding of structure function relationship to
the diseased state. Different Bioinformatics methodologies were
used to predict and compare the 3D structure of the wild-type and
its mutants. The predicted models were visualized by VMD, RasMol
and Pymol. Evaluation of the predicted models was performed using
DFIRE, Verify3D, ANOLEA and PROCHECK. Selected Model was compared
with its mutants and then simulated. MTHFR mutants were seen to
have decreased number of serine phosphorylation sites as compared
to wild-type. Phosphorylation seems to be playing a significant
role in function of this enzyme.
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