Aging occurs at the level of individual cells, a complex interplay between intrinsic "programming" and exogenous "wear and tear," with genetically-determined cellular capacity to repair environmentally-induced DNA damage playing a central role in the rate of aging and its specific manifestations. In 12 chapters, "The Role of DNA Damage and Repair in Cell Aging" provides an intellectual framework for aging of mitotic and post-mitotic cells, describes a variety of model systems for further studies, and reviews current concepts of DNA responses and their relationship to the phenomenon of aging.

As part of a series entitled "Advances in Cell Aging and Gerontology," this volume also summarizes seminal recent discoveries such as the molecular basis for Werner syndrome (a mutant DNA helicase), the complementary roles of telomere shortening and telomerase activity in cell senescence versus immortalization, the role of apoptosis in the homeostasis of aging tissue, and the existence of an inducible SOS-like response in mammalian cells that minimizes DNA damage from repeatedly encountered injurious environmental agents. Insights into the relationship between cellular aging and age-associated diseases, particularly malignancies, are also provided in several chapters.

This book is an excellent single source of information for anyone interested in DNA repair, mechanisms of aging, or certainly their intersection. Students will gain a general appreciation of these fields, but even the most senior investigators will benefit from the detailed coverage of rapidly advancing areas.

Immunoassay procedures (isotopic and non-isotopic) have become one of the single most important techniques in present-day diagnostic medicine. This book is designed as an introductory test for the staff of clinical research laboratories who conduct or intend to conduct such techniques, and will be of great value to the clinicians who make use of such services. The volume takes a three-pronged approach in it's in-depth presentation: explanation of the basic principles and applications of radioimmunoassays and non-isotopic immunoassays; practical illustrations of the various steps involved in immunoassays; discussion of the problems and pitfalls in immunoassays and how to avoid them. This fifth revised edition is a worthy successor to it's predecessors in this famous "Laboratory Techniques" series.

Part I covers modern advances in the determination of
glycoprotein structure and in the biosynthesis of mammalian,
bacterial, yeast, plant and insect glycoproteins. There are also
two chapters on functional aspects (glycoprotein hormones and
collagens).
The content of the volume is very comprehensive in that, most
contributors have focussed on discussing, in depth, the wealth
of most recent advances in their field, and referring to previous
reviews of older work for background information. This method can
effectively produce a very wide subject coverage in a smaller
number of chapters/volumes. The volume is an important
information source for all glycobiologist researchers (senior
investigators, post-doctoral fellows and graduate students), and
as a good, comprehensive, reference text for scientists working in
the life sciences.

Dry chemistry has been accepted as an important technology in medical laboratories for many years. Many evaluations of this technology have been undertaken by reputable clinical laboratories, the results of which were excellent when compared with conventional wet chemistry analysis.

This book contains a detailed overview of the current knowledge in the field of dry chemistry both in the physicians' office laboratories and large medical laboratories. The results from many evaluation studies are presented, as is data from interference studies which complete the descriptions of many dry chemistry methods.

A detailed description of various commercially available dry chemistry systems such as Ektachem, Reflotron, Seralyzer, Cobas Ready, Drichem, Opus and Stratus are also included.

This book effectively describes the current state-of-the-art technology and knowledge and succeeds in filling the gap in information in this important field of clinical chemistry science.

Originally published as 'Trockenchemie' by Georg Thieme Verlag,
Stuttgart, Dr. Sonntag has taken the opportunity of this translation to completely revise and update the contents of his book.

The series Practical Methods in Electron Microscopy, edited by Audrey M. Glauert has an international reputation as
a unique source of practical information for all electron microscopists.
Each book of the series starts from first principles, assuming no
specialist knowledge, and is complete in itself. The series will
eventually cover the whole range of techniques for electron microscopy.

In this latest volume in the popular series, Peter Lewis and David Knight describe in practical detail the whole range of staining techniques for electron microscopy, including contrast staining for ultrastructural studies, specific cytological staining methods, and enzyme cytochemical techniques.

The chemical basis of cytochemical methods is discussed and analysed in a straightforward manner with great clarity. In consequence this book will help the beginner to choose between the sometimes bewildering variety of published technical procedures.

Electron microscopists will find this volume to be an invaluable and compact source-book for all those cytochemical techniques that have proved to be both reliable and convenient to use. As with previous titles in this popular series, the unique type of practical guidance provided will be of value for many years to come.

Free radical species are generally short-lived due to their high reactivity and thus direct measurement and identification are often impossible. ESR is the only technique which has the potential for direct detection of radicals but in biological systems even these must be trapped by a spin-trapping agent. Thus most investigations involve recognition of indicators of the presence of radicals in vivo or "FOOTPRINTS" of radical-mediated damage.

"Techniques in Free Radical Research" assembles and critically assesses the most relevant and reliable experimental approaches used towards the measurement of radicals and radical-mediated damage in chemical systems, in cells and in tissues under the following six headings: a) Footprints of DNA damage, b) Footprints of protein damage, c) Footprints of lipid peroxidation, d) Footprints of antioxidant consumption, e) Footprints via indirect radical assays, and f) Footprints via the availability of transition metal complexes.

The central goal of this book is to broadly review the modem techniques and significant applications of chemical sensors and biosensors. Chapters are written by experts in the field - including Professor Joseph Wang, the most cited scientist in the world and renowned expert on sensor science who is also co-editor. Each chapter provides technical details beyond the level found in typical journal articles, and explores the application of chemical sensors and biosensors to a significant problem in biomedical science, also providing a prospectus for the future.
This book compiles the expert knowledge of many specialists in the construction and use of chemical sensors and biosensors including nitric oxide sensors, glucose sensors, DNA sensors, hydrogen sulfide sensors, oxygen sensors, superoxide sensors, immuno sensors, lab on chip, implatable microsensors, et al. Emphasis is laid on practical problems, ranging from chemical application to biomedical monitoring and from in vitro to in vivo, from single cell to animal to human measurement. This provides the unique opportunity of exchanging and combining the expertise of otherwise apparently unrelated disciplines of chemistry, biological engineering, and electronic engineering, medical, physiological.
The reader is not only provided with user-oriented guidelines for the proper choice and application of new chemical sensors and biosensors, but also with new methodological advancements related to and correlated with the measurement of interested species in biomedical samples. Many case studies are included to illustrate the range of application and importance of the chemical sensors and biosensors.

• Provides with user-orientedguidelines for the proper choice and application of new chemical sensors and biosensors
  
• Details new methodological advancements related to and correlated with the measurement of interested species in biomedical samples
  
• Contains many case studies to illustrate the range of application and importance of the chemical sensors and biosensors