Free radical species are generally short-lived due to their high
reactivity and thus direct measurement and identification are often
impossible. ESR is the only technique which has the potential for
direct detection of radicals but in biological systems even these
must be trapped by a spin-trapping agent. Thus most investigations
involve recognition of indicators of the presence of radicals in
vivo or "FOOTPRINTS" of radical-mediated damage.
"Techniques in Free Radical Research" assembles and critically
assesses the most relevant and reliable experimental approaches
used towards the measurement of radicals and radical-mediated
damage in chemical systems, in cells and in tissues under the
following six headings: a) Footprints of DNA damage, b) Footprints
of protein damage, c) Footprints of lipid peroxidation, d)
Footprints of antioxidant consumption, e) Footprints via indirect
radical assays, and f) Footprints via the availability of
transition metal complexes.
General
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