Protein NMR Spectroscopy combines a comprehensive theoretical treatment of NMR spectroscopy with an extensive exposition of the experimental techniques applicable to proteins and other biological macromolecules in solution. Beginning with simple theoretical models and experimental techniques, Protein NMR Spectroscopy develops the complete repertoire of theoretical principles and experimental techniques necessary for understanding and implementing the most sophisticated NMR experiments.
Important new techniques and applications of NMR spectroscopy have emerged since the first edition of this extremely successful book was published in 1996. The second edition includes new sections describing measurement and use of residual dipolar coupling constants for structure determination, TROSY and deuterium labeling for application to large macromolecules, and experimental techniques for characterizing conformational dynamics. In addition, the treatments of instrumentation and signal acquisition, field gradients, multidimensional spectroscopy, and structure calculation are updated and enhanced.
Protein NMR Spectroscopy is written as a graduate-level textbook and will be of interest to biochemists, chemists, biophysicists, and structural biologists who utilize NMR spectroscopy or who wish to understand the latest developments in this field.
. Provides an understanding of the theoretical principles important for biological NMR spectroscopy
. Demonstrates how to implement, optimize and troubleshoot modern multi-dimensional NMR experiments
. Allows for the capability of designing effective experimental protocols for investigations of protein structures and dynamics
. Includes a comprehensive set of example NMR spectra of ubiquitin provides a reference for validation of experimental methods"

Volume 1 of the series "Fundamentals of Medical Cell Biology" is devoted to evolutionary biology. This is presented in two parts: in the first, the structure and dynamics of RNA, DNA, and protein are dealt with. The second part is concerned with the origins and cellular basis of life.

The purpose of this volume is to provide a synopsis of present knowledge of the structure, organisation, and function of cellular organelles with an emphasis on the examination of important but unsolved problems, and the directions in which molecular and cell biology are moving. Though designed primarily to meet the needs of the first-year medical student, particularly in schools where the traditional curriculum has been partly or wholly replaced by a multi-disciplinary core curriculum, the mass of information made available here should prove useful to students of biochemistry, physiology, biology, bioengineering, dentistry, and nursing.
It is not yet possible to give a complete account of the relations between the organelles of two compartments and of the mechanisms by which some degree of order is maintained in the cell as a whole. However, a new breed of scientists, known as molecular cell biologists, have already contributed in some measure to our understanding of several biological phenomena notably interorganelle communication. Take, for example, intracellular membrane transport: it can now be expressed in terms of the sorting, targeting, and transport of protein from the endoplasmic reticulum to another compartment.
This volume contains the first ten chapters on the subject of organelles. The remaining four are in Volume 3, to which sections on organelle disorders and the extracellular matrix have been added.

Volume 1 of the series "Fundamentals of Medical Cell Biology" is devoted to evolutionary biology. This is presented in two parts: in the first, the structure and dynamics of RNA, DNA, and protein are dealt with. The second part is concerned with the origins and cellular basis of life.

Since the publication of the first edition of Regenerative Biology and Medicine in 2006, steady advances have been made in understanding the origin and characteristics of stem cells in epithelia, skeletal muscle, and bone, and in the niche signals that regulate the activities of these cells. Simultaneously, breakthroughs including the creation of iPSCs and transdifferentiation have created a momentum for regenerative biology with implications in regenerative biology that are far-reaching.

This book highlights these advances in the field to embrace a vast audience of investigators in chemistry, computer science, informatics, physics and mathematics as well as graduate students, clinical physicians, and biologists who are realizing the importance of the fields of regenerative biology and medicine in practice. Organized in three parts - biology of regeneration, regenerative medicine, and perspectives - this second edition creates a framework for integrating old and new data in this progressive field.
Includes coverage odskin, hair, teeth, cornea, and central neural tissuesProvides description of regenetive medicine indigestive, respiratory, urogenital, musculoskeletal, and cardiovascular systemsIncludes amphibians as powerful research models with discussion ofappendage regeneration in amphibians and mammals"

This manual is an indispensable tool for introducing advanced undergraduates and beginning graduate students to the techniques of recombinant DNA technology, or gene cloning and expression. The techniques used in basic research and biotechnology laboratories are covered in detail. Students gain hands-on experience from start to finish in subcloning a gene into an expression vector, through purification of the recombinant protein.
The second edition has been completely re-written, with new laboratory exercises and all new illustrations and text, designed for a typical 15-week semester, rather than a 4-week intensive course. The project approach to experiments was maintained: students still follow a cloning project through to completion, culminating in the purification of recombinant protein. It takes advantage of the enhanced green fluorescent protein-students can actually visualize positive clones following IPTG induction.
*Cover basic concepts and techniques used in molecular biology research labs
*Student-tested labs proven successful in a real classroom laboratories
*Exercises simulate a cloning project that would be performed in a real research lab
*"Project" approach to experiments gives students an overview of the entire process
*Prep-list appendix contains necessary recipes and catalog numbers, providing staff with detailed instructions"

Calcium is a versatile carrier of signals regulating many aspects of cellular activity such as fertilization to create a new life and programmed cell death to end it. Calcium homeostasis is strictly controlled by channels, pumps and exchangers functioning as gates for calcium entry and release. Given that calcium is such a versatile messenger the field of calcium signaling is continuously and rapidly expanding. This book reviews the most recent developments in calcium signaling by leading experts in the field. It is a state-of-the-art summary of our present knowledge in this quickly growing field and provides insight into the impressive progress made in many areas of calcium signaling, while reminding us of how much remains to be learned.

Caveolae (latin for little caves) are small structures found at the surface of cells. They are responsible for the regulation of important metabolic pathway. As a consequence, they may play a critical role in several human diseases such as atherosclerosis, cancer, diabetes, and muscular dystrophies. This book analyzes the role and function of caveolae in these aspects and serves as the first textbook currently available on caveolae/caveolin.

Molecular Diagnostics covers current molecular biological techniques used to identify the underlying molecular defects in inherited disease. Although an increasing number of laboratories, both academic and private are moving in that direction, there are only a few books in the existing literature, and they deal only partly with diagnosis at the molecular level. Each chapter includes the principle and a brief description of the technique, followed by exmples from the authors' own epertise. Contributors are well-known experts in their field, and derive from a variety of disciplines, to ensure breadth and depth of coverage.
* Examines widely used molecular biology techniques to screen for genetic defects causing inherited disorders
* Includes state-of-the-art techniques for the detection of the underlying genetic heterogeneity leading to inherited disorders
* Identification of genetically modified organisms (GMO's)
* Forensic analysis and every-day issues in a diagnostic laboratory
* Discusses ethics, genetic counselling and quality management

This book is the latest volume in a highly successful series within Comprehensive Biochemistry and provides a historical and autobiographical perspective of the development of the field through the contributions of leading individuals who reflect on their careers and their impact on biochemistry. The book is essential reading for everybody, from graduate student to professor, placing in context major advances not only in biochemical terms but in relation to historical and social developments. Readers will be delighted by the lively style and the insight into the lives and careers of leading scientists of their time.

This is the first book covering all aspects of high pressure biochemistry and biophysics of proteins.

Hydrostatic pressure is a powerful tool for study of biological systems. As a thermodynamic parameter, hydrostatic pressure has been known for a century to act on biological materials in a similar, but not identical, way to temperature. However, pressure was disregarded for a long time by biochemists mainly because the basic concepts (and the thermodynamics) focused on the chemical reactions involved and because general ideas on what pressure can add to the understanding of the behaviour of proteins were lacking. In recent decades, technological progress in the field of physics has shown, along with parameters such as temperature and solvent conditions, that pressure can be used for more refined thermodynamic and kinetic descriptions of biological processes and regulation of biological systems. The effects of pressure on proteins, nucleoproteins and membranes have recently been reviewed and several proceedings books have been published.

Drawing together literature from a variety of fields, Food Texture and Viscosity, 2E, includes a brief history of this area and its basic principles. It reviews how texture and viscosity are measured, including the physical interactions between the human body and food, objective methods of texture measurements, the latest advances in texture-measuring instruments, various types of liquid flow, and more.
This revised edition contains approximately 30% new material, including two new chapters on physics and texture and the correlation between physical measurements and sensory assessments. It now includes two-color illustrations and includes a current list of equipment suppliers.

* Completely revised with approximately 30% new material
* Includes two new chapters on physics and texture and the correlation between physical measurements and sensory assessments
* Provides a list of suppliers of texture-measuring equipment
* Features two-color illustrations and text throughout
* Written by an award-winning author

This is the third edition of an established and successful university textbook. The original structure and philosophy of the book continue in this new edition, providing a genuine synthesis of modern ecological and physiological thinking, while entirely updating the detailed content. New features include a fresh, unified treatment of toxicity, emphasizing common features of plant response to ionic, gaseous, and other toxins, explicit treatment of issues relating to global change, and a section on the role of fire in plant physiology and communities. The illustrations in the text are improved over previous editions, including color plates for the first time, and the authors' continuing commitment to providing wide citation of the relevant literature has further improved the reference list. This revision of Environmental Physiology of Plants will ensure the reputation of this title as a useful and relevant text well into the 21st century.
Key Features
* Includes enhanced illustrations, now with color plates
* Examines new molecular approaches which can be harnessed to solve problems in physiology
* Features new topics such as the unified treatment of toxicity, an explicit treatment of the issues relating to global change, and a section on the role of fire

This newest edition to the Laboratory Techniques Series gives current state of the art use of synthetic peptides in molecular biology and practical protocols on how to conjugate peptides, immunize animals with peptides and monitor immune responses to peptides in vitro.
It gives background information on antigenic specificity, prediction of antigenic sites in proteins and applications of peptides in immunology and virology, as probes in diagnosis and as vaccines. The book also describes antigenicity of proteins and methods to localize antigenic sites as well as methods for predicting epitoxes, and gives detailed protocols for peptide-carrier conjugation, immunization with peptides, and peptide immunoassays.
The volume also describes typical use of antipeptide antibodies in molecular and cellular biology as well as the use of peptides in the diagnosis of viral infections and autoimmune diseases, and the use of peptides as potential synthetic vaccines. An excellent edition to an excellent series, available in hardbound and paperback.

The central role of RNA in many cellular processes, in biotechnology, and as pharmaceutical agents, has created an interest in experimental methods applied to RNA molecules. This book provides scientists with a comprehensive collection of thoroughly tested up-to-date manuals for investigating RNA-protein complexes "in vitro." The protocols can be performed by researchers trained in standard molecular biological techniques and require a minimum of specialized equipment. The procedures include recommendation of suppliers of reagents.

The elucidation during the latter half of the 20th century of the mechanisms by which information flows from nucleic acids to proteins has completely changed the face of biological research. Many diseases are caused by abnormalities in control mechanisms which are not immediately essential for life itself but which maintain the normal social behavior of differentiated cells in multicellular organisms. The complex sugar chains of glycoproteins and glycolipids are believed to play important roles in the control of cellular functions and in recognition between the cell and its cellular and fluid environment. Investigations into the abnormalities of complex sugar chain assembly are expected to yield an important new underatanding of the etiology and pathogenesis of human diseases. This volume discusses several representative diseases which emphasize the current status of glycopathology, and will stimulate further research in this exciting field.

This publication contains full papers of both oral and poster presentations of the symposium "Immobilized Cells: Basics and Applications" that was held in Noordwijkerhout, The Netherlands, 26-29 November 1995. This volume covers recent developments in the field of immobilization e.g.: new support materials, characterization of support materials, kinetic characterizations, dynamic modelling, bioreactor types, scale up and applications are also given. Applications in the field of medicine, fermentation technology, food technology and environmental technology are described. Guidelines for research with immobilized cells. Based on the scientific sessions a strategy of research and methods for characterization of immobilized cells, especially in view of applications are given.

The goal was to relate basic research to applications and to extract guidelines for characterization of immobilized cells in view of process design and application from the contributions. The manuscripts presented in these proceedings give an extensive and recent overview of the research and applications of immobilized-cell technology.

Recent years have seen a considerable emphasis on growth factors and the elucidation of their mode of function, which has led to the recognition that growth factors, their receptors as well as downstream elements of signalling associated with their function might be potential targets in therapeutic management of human diseases. Humanised monoclonal antibodies raised against growth factor receptors have proved to be valuable for targeted cancer treatment and in patient management.

This book reviews the latest developments providing insights into the signalling processes involved in morphogenesis and pathogenesis with emphasis on using the elements of the signalling cascades as targets for therapeutic deployment.

Provides afundamental understanding of the basic functions of growth factors and their receptors, describing how they are linked in biological processes

Aids the development of therapeutic treatments for cancer

Focuses on the interrelationships and convergence of growth factors and their receptors in development and pathogenesis and encourages greater cooperation and integration in the areas of developmental, cancer and cancer therapeutic research "

"Designing Science Presentations "guides researchers and graduate students of virtually any discipline in the creation of compelling science communication. Most scientists never receive formal training in the creation, delivery, and evaluation of such material, yet it is essential for publishing in high-quality journals, soliciting funding, attracting lab personnel, and advancing a career.

This clear, readable volume fills that gap and provides visually intensive guidance at every step-from the construction of original figures to the presentation and delivery of those figures in papers, slideshows, posters, and websites. It provides pragmatic advice on the preparation and delivery of exceptional scientific presentations; demonstrates hundreds of visually striking presentation techniques, giving readers inspiration for creating their own; and is structured so that readers can easily find answers to particular questions.
Clear heading for each section indicates its message, highlighted with graphic illustrations Two summary paragraphs that complement the visual images and clearly discuss the main pointNumerous examples of high-quality figures, page layouts, slides, posters, and web pages to help stimulate readers' ideas for their own presentationsNumerous "before and after" examples to illustrate the contrast between poor and outstanding presentations

The accelerated globalization of the food supply, coupled with toughening government standards, is putting global food production, distribution, and retail industries under a high-intensity spotlight. High-publicity cases about foodborne illnesses over recent years have heightened public awareness of food safety issues, and momentum has been building to find new ways to detect and identify foodborne pathogens and eliminate food-related infections and intoxications. This extensively revised 4e covers how the incidence and impact of foodborne diseases is determined, foodborne intoxications with an introduction noting common features among these diseases and control measures that are applicable before and after the basic foodstuff is harvested.
Providesa summary of the"

The functional properties of any molecule are directly related to, and affected by, its structure. This is especially true for DNA, the molecular that carries the code for all life on earth.
The third edition of Understanding DNA has been entirely revised and updated, and expanded to cover new advances in our understanding. It explains, step by step, how DNA forms specific structures, the nature of these structures and how they fundamentally affect the biological processes of transcription and replication.
Written in a clear, concise and lively fashion, Understanding DNA is essential reading for all molecular biology, biochemistry and genetics students, to newcomers to the field from other areas such as chemistry or physics, and even for seasoned researchers, who really want to understand DNA.
* Describes the basic units of DNA and how these form the double helix, and the various types of DNA double helix
* Outlines the methods used to study DNA structure
* Contains over 130 illustrations, some in full color, as well as exercises and further readings to stimulate student comprehension

An important and comprehensive review of an expanding research area. The book will combine all classical knowledge in the field with recent advances to provide a full and comprehensive coverage of the field.
Transcription factors are important in regulating gene expression, and their analysis is of paramount interest to molecular biologists studying this area. This book looks at the basic machinery of the cell involved in transcription in eukaryotes, the factors involved in transcription and progresses to look at the regulatory systems which control this machinery both within the cell and also in the wider systems of the mammalian organism.
Key Features
* Comprehensive review of an increasingly important subject area
* Editor is well-known in this area, and has gathered a team of respected international contributors
* A unique collection of all recent work in this area, with no existing competition
* Covers both transcription factors and their control, and also both normal and disease states

Presents the principles of human gene evolution in a concise and easy to understand fashion. Uses examples of how evolutionary processes have molded present day genes, drawn from the evolution of humans and other primates, as well as from more primitive organisms. With increasing attention in this expanding area, this review forms a timely publication of our current knowledge of this important field.
Key Features
* Structure and function in the human genome
* The evolution of gene structure
* Mutational mechanisms in evolution

Nitric oxide (NO) is a gas naturally found in the body that conveys information between cells. In the last decade researchers have found that NO is a signaling molecule of key importance for the cardiovascular system, regulating blood pressure and blood flow to different organs. In addition, discoveries surrounding nitric oxide s role as a principal neurotransmitter moderating erectile function, a pathophysiological negotiator and messenger in inflammation, and a weapon against infections have increased research attention across the fields of biochemistry, chemistry, molecular biology, gene therapy, cell biology, immunology, pharmacology, neuroscience, and physiology.
Edited by Nobel Laureate Louis J. Ignarro

Up-to-date therapeutic implications of nitric oxide research Authored by world experts on nitric oxide Detailed research of the biochemistry and synthesis of nitric oxide

The clinical picture of diseases, caused by trinucleotide repeats, such as fragile X syndrome, myotonic dystrophy, Huntington's disease and forms of spinocerebella ataxia, provide the starting point for this authoritative review volume.
The book proceeds to integrate the current understanding of the molecular pathologies of these diseases, their genotype-phenotype relationships, the mutational processes involved and the laboratory and clinical issues relating to genetic testing for these disorders.
Clinicians and researchers in genetics, neuroscience, pediatrics and psychiatry will all benefit from the comprehensive overviews contributed by recognized world experts.