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Protein NMR Spectroscopy - Principles and Practice (Hardcover, 2nd edition)
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Protein NMR Spectroscopy - Principles and Practice (Hardcover, 2nd edition)
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Protein NMR Spectroscopy combines a comprehensive theoretical
treatment of NMR spectroscopy with an extensive exposition of the
experimental techniques applicable to proteins and other biological
macromolecules in solution. Beginning with simple theoretical
models and experimental techniques, Protein NMR Spectroscopy
develops the complete repertoire of theoretical principles and
experimental techniques necessary for understanding and
implementing the most sophisticated NMR experiments.
Important new techniques and applications of NMR spectroscopy have
emerged since the first edition of this extremely successful book
was published in 1996. The second edition includes new sections
describing measurement and use of residual dipolar coupling
constants for structure determination, TROSY and deuterium labeling
for application to large macromolecules, and experimental
techniques for characterizing conformational dynamics. In addition,
the treatments of instrumentation and signal acquisition, field
gradients, multidimensional spectroscopy, and structure calculation
are updated and enhanced.
Protein NMR Spectroscopy is written as a graduate-level textbook
and will be of interest to biochemists, chemists, biophysicists,
and structural biologists who utilize NMR spectroscopy or who wish
to understand the latest developments in this field.
. Provides an understanding of the theoretical principles important
for biological NMR spectroscopy
. Demonstrates how to implement, optimize and troubleshoot modern
multi-dimensional NMR experiments
. Allows for the capability of designing effective experimental
protocols for investigations of protein structures and
dynamics
. Includes a comprehensive set of example NMR spectra of ubiquitin
provides a reference for validation of experimental methods"
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