"Alberto Diaspro has been choreographing light's dance for over 20
years, and in Nanoscopy and Multidimensional Optical Fluorescence
Microscopy, he has assembled a diverse group of experts to explain
the methods they use to coax light to reveal biology's secrets." -
From the Foreword by Daniel Evanko, editor, Nature Methods
Nanoscopy and Multidimensional Optical Fluorescence Microscopy
demonstrates that the boundaries between sciences do blur at the
bottom, especially those that might separate the optical work of
physicists and the cellular work of microbiologists. In 18 chapters
written by pioneering researchers, this work offers the first
comprehensive and current documentation of the cutting-edge
research being accomplished in a wide range of photonic devices
with revolutionary application. The highlight of the book is its
coverage of optical nanoscopy and super-resolution microscopy. The
rapid advances in this area over the past few years offer
researchers in both photonics and molecular biologya wealth of
accomplishment upon which they can build. Offering a complete
treatment of this emerging field, this volume: Describes how
scientists have exploited the properties of light and its
fluorophore partners to overcome the resolution limit of
conventional light microscopy Delves into recent ways to minimize
the photobleaching that has long hampered many methods including
those that have the potential to capture previously unobtainable
information on the movements of single molecules Discusses the
principles, benefits, and implementation of fluorescence
correlation spectroscopy and related methods, which simplifies
analysis by limiting light to stationary focal points in a sample
Considers the most basic as well as emerging methods for improving
three-dimensional optical sectioning microscopy Reviews the basics
of FRET (
General
Imprint: |
Crc Press
|
Country of origin: |
United Kingdom |
Release date: |
October 2019 |
First published: |
2009 |
Editors: |
Alberto Diaspro
|
Dimensions: |
254 x 178mm (L x W) |
Format: |
Paperback
|
Pages: |
448 |
ISBN-13: |
978-0-367-38421-0 |
Categories: |
Books >
Science & Mathematics >
Physics >
Optics (light)
|
LSN: |
0-367-38421-3 |
Barcode: |
9780367384210 |
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