Common (bread) wheat is an allohexaploid species (Triticum aestivum
L.) with 3 compensating genomes AABBDD (2n=6x=42 chromosomes).
Traditionally genetic and physical mapping in this most important
cereal crop has been based on molecular markers (RFLPs, PCR, Insitu
hybridization etc). The recent development in producing deletion
lines of common wheat (using Aegilops cylindricum genome) has
revolutionized the physical mapping of wheat chromosomes. During
present study, deletion lines of chromosomes 5A of common wheat
were used to identify RAPD (Randomly Amplified Polymorphic DNA)
based molecular markers specific for short and/or long arm of
chromosome 5A. Out of the 7 RAPD primers used, OPA-07 showed useful
polymorphism for long arm deletion lines viz; del5AL-10 and
del5AL-23. By comparing the C-banding karyotype of the deletion
lines, it is inferred that the primer (OPA-07) anneals to the
distal half of the long arm of chromosome 5A.
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