Molecular biology has revolutionized research into vascular
disease. Over the past 20 years molecular techniques have enabled
us to both elucidate - lecular mechanisms in vascular disease and
identify appropriate therapies. The vast explosion in technical
knowledge and the array of protocols that become more advanced and
intricate by the day lead us into new and exciting areas of
research that were previously unobtainable. Vascular Disease:
Molecular Biology and Gene Transfer Protocols - scribes today's
most powerful molecular methods for the investigation of the
pathogenesis of vascular disease. The protocols are highly
detailed, allowing beginners who have little experience in either
vascular biology or molecular biology to embark on new molecular
projects. This book is also suited to more experienced molecular
biologists who wish to grasp new methods for stu- ing the
involvement of genes in normal vascular physiology and in diseased
states. It is well established that cardiovascular disease
progression has a s- stantial genetic influence. Part I describes
three methods that have been used successfully to identify specific
mutations in candidate genes involved in c- diovascular disorders.
These mutations include both single-stranded conf- mational
polymorphism analysis and heteroduplex detection methods. In
addition, technology to map new genes to specific regions of
chromosomes by high-resolution mapping is described.
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