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Vaccine Protocols (Hardcover, 2nd ed. 2003)
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Vaccine Protocols (Hardcover, 2nd ed. 2003)
Series: Methods in Molecular Medicine, 87
Expected to ship within 10 - 15 working days
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Vaccine research and development is advancing at an unprecedented
pace, with an increasing emphasis on rational design based upon a
fundamental und- standing of the underlying molecular mechanisms.
The aim of this volume is to provide a selection of contemporary
protocols that will be useful to both novice and advanced
practitioner alike. The variety of procedures required to design,
develop, produce, and assess a vaccine is immense and covers
aspects of ch- istry, biochemistry, molecular biology, cell
biology, and immunology. No single volume can hope to cover these
topics exclusively. Rather, here we attempt to provide a methods
sourcebook focusing on hands-on practical advice. Comp- mentary and
background information may be found in other volumes in the Me- ods
in Molecular Medicine series. Of particular interest are volumes on
Dendritic Cell Protocols, Interleukin Protocols, Vaccine Adjuvants,
and DNA Vaccines. Since the publication of the first edition of
Vaccine Protocols there have been major advances, particularly in
the areas of bacterial genomics, antig- specific T-cell
quantification, genetic manipulation of vaccine vectors, the h-
nessing of natural molecules concerned with the regulation of
immune responses, and the burgeoning field of DNA vaccinology.
Hence, the extensive revision of this edition with new chapters on
live viral vaccine vectors, atte- ated bacterial vectors,
immunomodulators, MHC-peptide tetrameric complexes, and the
identification of vaccine candidates by genomic analysis.
Additionally, chapters from the first edition have been updated to
accommodate state-of-t- art methods in vaccinology.
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