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Pollen and Pollen Tube Biology - Methods and Protocols (Hardcover, 1st ed. 2020)
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Pollen and Pollen Tube Biology - Methods and Protocols (Hardcover, 1st ed. 2020)
Series: Methods in Molecular Biology, 2160
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This volume explores a collection of experimental techniques used
to investigate different aspects of pollen development and
function, including its role in reaching the ovule and delivering
the two sperm cells. The techniques discussed range from basic
methodology to cultivate pollen in vitro to the sophisticated
experiments involving micromanipulation, Lab-on-Chip technology, or
high-end imaging. The chapters in this book cover topics such as
pollen grain counting using a cell counter; restricted pollination
for tracing individual pollen tubes in a pistil; obtaining mutant
pollen for phenotypic analysis and pollen tube dual-staining;
analyzing intracellular gradients in pollen tubes; and measuring
exocytosis rate in Arabidopsis pollen tube using corrected
fluorescence recovery after photoconversion (cFRAPc) technique.
Written in the highly successful Methods in Molecular Biology
series format, chapters include introductions to their respective
topics, lists of the necessary materials and reagents,
step-by-step, readily reproducible laboratory protocols, and tips
on troubleshooting and avoiding known pitfalls. Cutting-edge and
thorough, Pollen and Pollen Tube Biology: Methods and Protocols is
a valuable resource for novice and expert scientists interested in
learning more about the field of plant reproduction.
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