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Books > Science & Mathematics > Biology, life sciences > Biochemistry > Proteins
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Receptor Binding Techniques (Hardcover, 3rd ed. 2012)
Loot Price: R2,999
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Receptor Binding Techniques (Hardcover, 3rd ed. 2012)
Series: Methods in Molecular Biology, 897
Expected to ship within 10 - 15 working days
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A broad definition of a receptor is a specialized protein on or in
a cell that recognizes and binds a specific ligand to undergo a
conformational change, leading to a physiological response or
change in cell function. A ligand can be an endogenous
neurotransmitter, hormone, paracrine/autocrine factor, or a
synthetic drug that may function as an agonist or antagonist. The
third edition of Receptor Binding Techniques expands upon the
methods and techniques used for studying receptors in silico, in
vitro and in vivo. Comprehensive chapters describe how to use
online resources for experimental research such as prediction of
receptor-ligand interactions and mine the IUPHAR receptor database.
Classical techniques of radioligand binding, quantitative
autoradiography and their analyses are complemented by the use of
immunocytochemistry for the cellular localization of receptor
protein and hybridization to detect receptor mRNA. Protocols using
fluorescent labeled ligands are described to visualise receptors in
living cells, their interaction with beta-arrestin to measure
ligand-induced internalisation and green fluorescent protein to
study trafficking. Non-radioactive, chemiluminescent cAMP and
arrestin assays facilitate the identification of novel 'biased
agonists'. Detailed methods are provided for in vivo imaging of
receptors using positron emission tomography (PET). Written in the
highly successful Methods in Molecular Biology (TM) series format,
chapters include introductions to their respective topics, lists of
the necessary materials and reagents, step-by-step, readily
reproducible laboratory protocols, and key tips on troubleshooting
and avoiding known pitfalls. Authoritative and practical, Receptor
Binding Techniques, Third Edition, aids scientists in continuing to
study receptor binding.
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