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Electroporation and Electrofusion in Cell Biology (Hardcover, 1989 ed.)
Loot Price: R5,976
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Electroporation and Electrofusion in Cell Biology (Hardcover, 1989 ed.)
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Cells can be funny. Try to grow them with a slightly wrong recipe,
and they turn over and die. But hit them with an electric field
strong enough to knock over a horse, and they do enough things to
justify international meetings, to fill a sizable book, and to lead
one to speak of an entirely new technology for cell manipulation.
The very improbability of these events not only raises questions
about why things happen but also leads to a long list of practical
systems in which the application of strong electric fields might
enable the merger of cell contents or the introduction of alien but
vital material. Inevitably, the basic questions and the practical
applications will not keep in step. The questions are intrinsically
tough. It is hard enough to analyze the action of the relatively
weak fields that rotate or align cells, but it is nearly impossible
to predict responses to the cell-shredding bursts of electricity
that cause them to fuse or to open up to very large molecular
assemblies. Even so, theoretical studies and systematic examination
of model systems have produced some creditable results, ideas which
should ultimately provide hints of what to try next.
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