This volume explores experimental approaches used to study Duchenne
muscular dystrophy (DMD), an X-linked degenerative skeletal muscle
disease caused by mutations in the dystrophin gene. Including the
latest progress and scientific achievements, the book covers recent
discoveries achieved through in vivo gene editing which have proven
to be promising in restoring dystrophin expression, at least in
ameliorating skeletal muscle symptoms, and the contents focus on
"Omics" techniques in gene expression, protein expression, miRNAs,
and long non-coding RNA analysis, as well as experimental studies
of the structural/functional changes affecting the skeletal and
cardiac muscles and ongoing preclinical studies and clinical
trials. Written in the highly successful Methods in Molecular
Biology series format, chapters include introductions to their
respective topics, lists of the necessary materials and reagents,
step-by-step, readily reproducible laboratory protocols, and tips
on troubleshooting and avoiding known pitfalls. Authoritative and
practical, Duchenne Muscular Dystrophy: Methods and Protocols
serves as a guide for researchers exploring the complicated nature
of dystrophin in the hope of helping the victims of this disorder.
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