METHODS IN PLANT HISTOLOGY - PREFACE TO THE FIRST EDITION -This
book has grown out of a course in histological technic conducted by
the author at the University of Chicago. The course has also been
taken by non-resident students through the Extension Division of
the University. The Methods were published over a year ago as a
series of articles in the Journal of Applied Microscopy, and have
called out numerous letters of commendation, criticism, suggestion,
and inquiry. The work has been thoroughly revised and enlarged by
about one-half. It is hoped that the criticism and suggestion, and
also the experience gained by contact with both resident and
non-resident students, have made the directions so definite that
they may be followed, not only by those who work in a class under
the supervision of an instructor, but also by those who must work
in their own homes without any such assistance. More space has been
devoted to the paraffin method than to any other, because it has
been proved to be better adapted to the needs of the botanist. The
celloidin method, the glycerin method, and freehand sectioning are
also described, and their advantages and disadvantages are pointed
out. The first part of the book deals with the principles of king
and staining, and the various other processes of microtechnic,
while in the later chapters these principles are applied to
specific cases. This occasions some repetition, but the mere
presentation of general principles will not enable the beginner to
make good mounts. The illustrations and notes in the later chapters
are not intended to afford a study of general morphology, but they
merely indicate to students with a limited knowledge of plant
structures theprincipal v PREFACE TO THE THIRD EDITION The
continued appreciation accorded to Methods in Plant Histology has
exhausted the second edition. Since that edition appeared, methods
have become more and more exact, so that the present volume is
practically a new book. The general arrangement of the
subject-matter, and directions for collecting material and for
securing reproductive phases in the Algae and Fungi have been
retained, and a chapter on Photomicrographs and Lantern Slides
chap. xii his been added. Great improvements have been made . in
the paraffin method, so that sections are easily cut which were
impossible ten years ago, while ten years of added experience with
the Venetian turpentine method have made it possible to describe it
so definitely that even the beginner should find no serious
difficulty. The author is deeply indebted to his colleague, Dr. TV.
J. G. Land, for numerous suggestions and improvements covering the
whole field of microtechnic. He is also greatly indebted to Dr. S.
Yamanouchi for many improvements in the methods applicable to Algae
and mitotic figures. Corrections and suggestions will be heartily
appreciated. CHICAGO May, 1915 I. RT I PAGE CH. IPTER I1 . RE. IC.
ESTS . . . . . . . . . 1 7 Killing and Fixing Ige lts . . . . . . .
. . . . . 17 Dehydrating - 1gcnts . . . . . . . . . . . 31 Formulae
for, l lcohols . . . . . . . . . . 33 Clearing- gents . . . . . . .
. . . . . . . . 34 1Iisccllaneous Iieagerlt s . . . . ...
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