Leading drosophilists describe in step-by-step detail all the
essential techniques for studying Drosophila chromosomes and
suggest new avenues for scientific exploration. The chapters
emphasize specimen preparation (from dissection to mounting) and
cover both polytene and mitotic/meiotic chromosomes in depth. Each
fully tested and readily reproducible protocol offers a background
introduction, equipment and reagent lists, and tips on
troubleshooting and avoiding pitfalls. A cutting-edge FISH and
immunolocalization technique will be important for discovering how
DNA sequence influences higher-order chromosome architecture and
ultimately gene expression.
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