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Plant Circadian Networks - Methods and Protocols (Hardcover, 2014 ed.)
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Plant Circadian Networks - Methods and Protocols (Hardcover, 2014 ed.)
Series: Methods in Molecular Biology, 1158
Expected to ship within 12 - 17 working days
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Plant Circadian Networks: Methods and Protocols provides a
collection of protocols to investigate clock-controlled parameters
including transcript and small RNA levels, promoter activity using
luciferase reporters, protein levels and posttranslational
modification, protein-protein interaction, in vivo DNA-protein
interaction and RNA-protein interaction, cellular redox state, Ca2+
levels, and innate immune responses. Furthermore, the use of
bioinformatics resources is described to evaluate high throughput
data sets and to integrate the data into an overarching picture of
circadian networks in the cell. Additional chapters focus on
seasonal processes like flowering time control, and techniques on
trees, moss and algae. Written in the highly successful Methods in
Molecular Biology series format, chapters include introductions to
their respective topics, lists of the necessary materials and
reagents, step-by-step, readily reproducible laboratory protocols,
and key tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical, Plant Circadian Networks: Methods and
Protocols is designed not only for the chronobiology community
dealing with circadian biology but also for the plant community in
general.
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