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Methods in Membrane Biology - Volume 3 Plasma Membranes (Paperback, Softcover reprint of the original 1st ed. 1975)
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Methods in Membrane Biology - Volume 3 Plasma Membranes (Paperback, Softcover reprint of the original 1st ed. 1975)
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Volume 3 continues the approach carried out in the first two
volumes of this se ries of publishing articles on membrane
methodology which include, in addition to procedural details,
incisive discussions of the ap plications of the methods and of
their limitations. Wh at is the theoretical basis of the method,
how and to what problems can it be applied, how does one interpret
the results, what has thus far been achieved by the method, what
lies in the future-these are the questions the authors have tried
to answer. No area of membrane biology engages the interest of more
investigators than studies of the plasma membrane. Four chapters in
this volume are concerned with one or more aspects of the cell
surface. Fundamental to all studies of the cell surface are the
isolation and characterization of pure plasma membranes. Many
preparations described in the literature are inadequate or are
inadequately characterized. In the first chapter, Neville discusses
the theoretical and practical bases of tissue fractionation, empha
sizes the variations in enzyme content among plasma membranes from
different sources, offers guidance in the choice of the proper
criteria for assessing membrane purity, and suggests the best
markers for detecting the possible presence of contaminating
organelles. To review in detail each of the many preparations of
plasma membranes that have been published is impossible.
General
Imprint: |
Springer-Verlag New York
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Country of origin: |
United States |
Release date: |
August 2014 |
First published: |
1975 |
Authors: |
Edward D. Korn
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Dimensions: |
229 x 152 x 14mm (L x W x T) |
Format: |
Paperback
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Pages: |
246 |
Edition: |
Softcover reprint of the original 1st ed. 1975 |
ISBN-13: |
978-1-4757-0267-5 |
Categories: |
Books >
Science & Mathematics >
Biology, life sciences >
Biochemistry >
General
|
LSN: |
1-4757-0267-1 |
Barcode: |
9781475702675 |
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