"Low-Abundance Proteome Discovery" addresses the most critical
challenge in biomarker discovery and progress: the identification
of low-abundance proteins. The book describes an original strategy
developed by the authorsthat permits the detection of protein
species typically found in very low abundance and that may yield
valuable clues to future discoveries. Known as combinatorial
peptide ligand libraries, these new methodologies are one of the
hottest topics related to the study of proteomics and have
applications in medical diagnostics, food quality, and plant
analysis. The book is written for university and industry
scientists starting proteomic studies of complex matrices (e.g.,
biological fluids, biopsies, recalcitrant plant tissues, foodstuff,
and beverage analysis), researchers doing wet chemistry, and
graduate-level students in the areas of analytical and
biochemistry, biology, and genetics.
Covers methodologies for enhancing the visibility of low-abundance
proteins which, until now, has been the biggest challenge in
biomarker progressIncludes detailed protocols that address
real-life needs in laboratory practiceAddresses all applications,
including human disease, food and beverage safety, and the
discovery of new proteins/peptides of importance in
nutraceuticsCompiles the research and analytic protocols of the two
scientists who are credited with the discovery of these landmark
methodologies, also known as combinatorial peptide ligand
libraries, for the identification of low-abundance proteins"
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