This book provides the first systematic treatment of the
thermodynamic theory of site-specific effects in biological
macromolecules. It describes the phenomenological and conceptual
bases required to allow a mechanistic understanding of these
effects from analysis of experimental data. The thermodynamic
theory also results in novel experimental strategies that enable
the derivation of information on local, site-specific properties of
a macromolecular system from analysis of perturbed global
properties. The treatment focuses on binding phenomena, but is
amenable to extension both conceptually and formally to the
analysis of other cooperative processes, such as folding and helix
coil transitions. This book will interest any scientist involved in
structure function studies of biological macromolecules, or as a
text for graduate students in biochemistry and biophysics.
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