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Single Molecule Analysis - Methods and Protocols (Paperback, Softcover reprint of the original 1st ed. 2011)
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Single Molecule Analysis - Methods and Protocols (Paperback, Softcover reprint of the original 1st ed. 2011)
Series: Methods in Molecular Biology, 783
Expected to ship within 10 - 15 working days
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Life scientists believe that life is driven, directed, and shaped
by biomolecules working on their own or in concert. It is only in
the last few decades that technological breakthroughs in sensitive
fluorescence microscopy and single-molecule manipulation techniques
have made it possible to observe and manipulate single biomolecules
and measure their individual properties. The methodologies
presented in Single Molecule Techniques: Methods and Protocols are
being applied more and more to the study of biologically relevant
molecules, such as DNA, DNA-binding proteins, and motor proteins,
and are becoming commonplace in molecular biophysics, biochemistry,
and molecular and cell biology. The aim of Single Molecule
Techniques: Methods and Protocols is to provide a broad overview of
single-molecule approaches applied to biomolecules on the basis of
clear and concise protocols, including a solid introduction to the
most widely used single-molecule techniques, such as optical
tweezers, single-molecule fluorescence tools, atomic force
microscopy, magnetic tweezers, and tethered particle motion.
Written in the highly successful Methods in Molecular Biology (TM)
series format, chapters contain introductions to their respective
topics, lists of the necessary materials and reagents,
step-by-step, readily reproducible laboratory protocols, and notes
on troubleshooting and avoiding known pitfalls. Authoritative and
accessible, Single Molecule Techniques: Methods and Protocols
serves as an ideal guide to scientists of all backgrounds and
provides a broad and thorough overview of the exciting and
still-emerging field of single-molecule biology.
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