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Directed Evolution Library Creation - Methods and Protocols (Hardcover, 2003 ed.)
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Directed Evolution Library Creation - Methods and Protocols (Hardcover, 2003 ed.)
Series: Methods in Molecular Biology, 231
Expected to ship within 10 - 15 working days
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Biological systems are very special substrates for
engineering-uniquely the products of evolution, they are easily
redesigned by similar approaches. A simple algorithm of iterative
cycles of diversification and selection, evolution works at all
scales, from single molecules to whole ecosystems. In the little
more than a decade since the first reported applications of
evolutionary design to enzyme engineering, directed evolution has
matured to the point where it now represents the centerpiece of
industrial biocatalyst development and is being practiced by
thousands of academic and industrial scientists in com- nies and
universities around the world. The appeal of directed evolution is
easy to understand: it is conceptually straightforward, it can be
practiced without any special instrumentation and, most important,
it frequently yields useful solutions, many of which are totally
unanticipated. Directed evolution has r- dered protein engineering
readily accessible to a broad audience of scientists and engineers
who wish to tailor a myriad of protein properties, including th-
mal and solvent stability, enzyme selectivity, specific activity,
protease s- ceptibility, allosteric control of protein function,
ligand binding, transcriptional activation, and solubility.
Furthermore, the range of applications has expanded to the
engineering of more complex functions such as those performed by m-
tiple proteins acting in concert (in biosynthetic pathways) or as
part of mac- molecular complexes and biological networks.
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