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Books > Science & Mathematics > Biology, life sciences > Cellular biology
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Germ Cell Protocols - Volume 2: Molecular Embryo Analysis, Live Imaging, Transgenesis, and Cloning (Hardcover, 2004 ed.)
Loot Price: R2,945
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Germ Cell Protocols - Volume 2: Molecular Embryo Analysis, Live Imaging, Transgenesis, and Cloning (Hardcover, 2004 ed.)
Series: Methods in Molecular Biology, 254
Expected to ship within 10 - 15 working days
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For volume 2 alone:
A gold-standard collection of readily reproducible techniques for
the molecular and genetic analysis of germ cells in a variety of
different reproductive systems. Volume 2: Molecular Embryo
Analysis, Live Imaging, Transgenesis, and Cloning focuses on
molecular egg analysis, live egg imaging, nuclear cloning, oocyte
cryopreservation, and nuclear transfer. Highlights include the
identification and characterization of Oct-4, germline chromatin
silencing by RNAi, cDNA subtraction and cloning in the field of
trophoblast/placental development, and selective ablation. Each
readily reproducible protocol is described in step-by-step detail
and contains an introduction outlining the principle behind the
technique, lists of equipment and reagents, and tips on
troubleshooting and avoiding known pitfalls. Comprehensive and
cutting-edge, Germ Cell Protocols offers both novice and
established researchers a gold-standard collection of hard-to-find
methods of high impact research, diverse procedures that are
easy-to-follow, well-illustrated, and allow a cross-species
transfer of knowledge from lower vertebrates to higher mammalian
systems.
For both volumes:
A gold-standard collection of readily reproducible techniques for
the molecular and genetic analysis of germ cells in a variety of
different reproductive systems. The methods cover sperm and egg
activation, motility, fertilization, nuclear development, nuclear
cloning, the molecular characterization of specific events, and the
imaging of cell structures. Volume 1: Sperm and Oocyte Analysis
focuses on sperm cells, oocyte analysis, oocyte maturation,
fertilization, and preparation techniques. Volume 2:
MolecularEmbryo Analysis, Live Imaging, Transgenesis, and Cloning
contains methods for molecular egg analysis, live egg imaging,
nuclear cloning, oocyte preservation, and nuclear transfer.
Comprehensive and cutting-edge, Germ Cell Protocols offers both
novice and established researchers a gold-standard collection of
hard-to-find methods of high impact research, diverse procedures
that are easy-to-follow, well-illustrated, and allow a
cross-species transfer of knowledge from lower vertebrates to
higher mammalian systems.
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