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Simultaneous Estimation of Antihypertensive Drugs by HPLC (Paperback)
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Simultaneous Estimation of Antihypertensive Drugs by HPLC (Paperback)
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Every year many new drugs and newer drug combinations enter the
pharmaceutical area. Analytical methods for these new and first
timer drugs are mostly confined only to the manufacturing company.
However, availability of multiple analytical methods for the same
drug/drug combinations in their formulations is always
advantageous. Moreover, development of such methods helps in
training the analysts for skillfully handling the sophisticated
analytical instruments and the way for research approach. The
present work is also planned on similar lines. A simple, precise,
rapid and accurate Reversed-Phase High Performance Liquid
Chromatography method is developed for the simultaneous estimation
of Amlodipine Besylate, Olmesartan Medoxomil and Hydrochlorthiazide
in dosage forms. Chromatography is carried out isocratically at 25
C 0.5 C on Liquid chromatographic system from Young Lin 9100
comprising of hyperchrome C-18 column (4.6 x 250mm, 5 particle
size), manual injector, YL 9110 quaternary pump and Photodiode
array YL 9160 detector connected to software YL clarity. Parameters
such as linearity, precision, accuracy, recovery and specificity
are studied as reported in the ICH guidelines.
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