The success of laboratory experiments relies heavily on the
technical ability of the bench scientist, with the aid of
"tricks-of-the-trade", to generate consistent and reliable data.
Regrettably, however, these invaluable "tricks-of-the-trade" are
frequently omitted from scientific publications. This paucity of
practical information relating to the conduct of laboratory
bacteriology experiments creates a gaping void in the pertinent
literature. Methods in Practical Laboratory Bacteriology fills this
void. It provides detailed technical information that ensures that
you achieve consistent and reliable data. The book addresses the
aspects of bacterial fractionation and membrane characterization,
the analysis of Lipopolysaccharides and the techniques of SDS-PAGE,
immunoblotting, and ELISA. It also describes the methods used for
detecting and quantifying bacterial resistance to antibiotics, and
the analysis of bacterial chromosomes by pulsed-field gel
electrophoresis (PFGE). Methods in Practical Laboratory
Bacteriology also covers protocols for extracting the
fingerprinting plasmids, as well as the use of non-radio labeled
gene probes and ribosomal RNA gene probes.
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