How one goes about analyzing proteins is a constantly evolving
field that is no longer solely the domain of the protein
biochemist. Investi gators from diverse disciplines find themselves
with the unanticipated task of identifying and analyzing a protein
and studying its physical properties and biochemical interactions.
In most cases, the ultimate goal remains understanding the role(s)
that the target protein is playing in cellular physiology. It was
my intention that this manual would make the initial steps in the
discovery process less time consuming and less intimidating. This
book is not meant to be read from cover to cover. The expanded
Table of Contents and the index should help locate what you are
seeking. My aim was to provide practically oriented information
that will assist the experimentalist in benchtop problem solving.
The appendices are filled with diverse information gleaned from
catalogs, handbooks, and manuals that are presented in a distilled
fashion designed to save trips to the library and calls to
technical service representatives. The user is encouraged to expand
on the tables and charts to fit individual experimental situations.
This second edition pays homage to the computer explosion and the
various genome projects that have revolutionized how benchtop
scientific research is performed. Bioinformatics and In silica
science are here to stay. However, the second edition still
includes recipes for preparing buffers and methods for lysing
cells."
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