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Books > Science & Mathematics > Biology, life sciences > Cellular biology
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Plant Cell Electroporation And Electrofusion Protocols (Paperback, 1995 ed.)
Loot Price: R2,367
Discovery Miles 23 670
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Plant Cell Electroporation And Electrofusion Protocols (Paperback, 1995 ed.)
Series: Methods in Molecular Biology, 55
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Gene transfer is an essential technology for improving our under
standing of gene structure and function. Although there are many
meth ods by which DNA may be introduced into cells-including heat
and chemical treatments, and microinjection-electroporation has
been found to be the most versatile gene transfer technique.
Electroporation is effective with a wide variety of cell types,
including those that are difficult to transform by other means. For
many cell types, electroporation is either the most efficient or
the only means known to effect gene transfer. The early and broad
success of electric field-medi ated DNA transfer soon prompted
researchers to investigate electroporation for transferring other
types of molecules into cells, in cluding RNA, enzymes, antibodies,
and analytic dyes. The first section of Plant Cell Electroporation
and Electrofusion Protocols includes two chapters that serve as a
guide to theoretical and practical aspects of electroporation, and
will be of particular interest to those developing protocols for as
yet untested species or cell types, and a third chapter that
describes commercially available electroporation instruments. The
remaining chapters describe well-tested protocols for DNA
electrotransfection, electroporation of other biomolecules, or cell
electrofusion. These chapters also include brief discussions of
alterna tives to electric field-based methods, citing the
advantages and limita tions of the various methods for achieving
specific goals."
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