The detection of genetically modified organisms (GMOs) is
becoming very complex, with new GMOs, approved and unapproved,
constantly entering world markets. Traceability and labelling of
GMOs is defined in regulations worldwide, demanding accurate and
reliable testing to support the requirements of legislation.
ThisBrief provides the current state-of-the-art on all key
topics involved in GMO testing and is a source of detailed
practical information for laboratories. Special focus is given to
qualitative and quantitative real-time PCR analysis relevant to all
areas where detection and identification rely on nucleic acid-based
methods. The following topics, important for testing laboratories,
are also discussed: organization of the laboratory, focusing on
aspects of the quality system and methods for testing, validation
and verification of methods, and measurement uncertainty. The Brief
also discusses the new challenges of GMOs and novel modified
organisms, using new technologies, and the possible solutions for
GMO detection, including bioinformatics tools. Finally, legislation
on GMOs and sources of information on GMOs are provided, which are
relevant not only to testing laboratories, but to anyone interested
in GMOs.
The authors of this Brief have many years of experience in GMO
testing, development of real-time PCR methods, implementation of
quality system requirements, validations and verification of
methods, and measurement uncertainty. The National Institute of
Biology is a highly qualified research laboratory and a National
Reference Laboratory, which also performs routine analyses of food,
feed and seed. The Institute for Health and Consumer Protection of
the European Union Joint Research Centre has extensive knowledge
and experience of GMO detection. It hosts the European Union
Reference Laboratory for GM Food and Feed in addition to chairing
the European Network of GMO Laboratories.
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