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Books > Science & Mathematics > Physics > Applied physics & special topics > Biophysics
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Membrane Proteins in Aqueous Solutions - From Detergents to Amphipols (Hardcover, 1st ed. 2018)
Loot Price: R6,366
Discovery Miles 63 660
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Membrane Proteins in Aqueous Solutions - From Detergents to Amphipols (Hardcover, 1st ed. 2018)
Series: Biological and Medical Physics, Biomedical Engineering
Expected to ship within 10 - 15 working days
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This book is the first to be entirely devoted to the challenging
art of handling membrane proteins out of their natural environment,
a key process in biological and pharmaceutical research, but one
plagued with difficulties and pitfalls. Written by one of the
foremost experts in the field, Membrane Proteins in Aqueous
Solutions is accessible to any member of a membrane biology
laboratory. After presenting the structure, functions, dynamics,
synthesis, natural environment and lipid interactions of membrane
proteins, the author discusses the principles of extracting them
with detergents, the mechanisms of detergent-induced
destabilization, countermeasures, and recent progress in developing
detergents with weaker denaturing properties. Non-conventional
alternatives to detergents, including bicelles, nanodiscs,
amphipathic peptides, fluorinated surfactants and amphipols, are
described, and their relative advantages and drawbacks are
compared. The synthesis and solution properties of the various
types of amphipols are presented, as well as the formation and
properties of membrane protein/amphipol complexes and the transfer
of amphipol-trapped proteins to detergents, nanodiscs, lipidic
mesophases, or living cells. The final chapters of the book deal
with applications: membrane protein in vitro folding and cell-free
expression, solution studies, NMR, crystallography, electron
microscopy, mass spectrometry, amphipol-mediated immobilization of
membrane proteins, and biomedical applications. Important features
of the book include introductory sections describing foundations as
well as the state-of-the-art for each of the biophysical techniques
discussed, and topical tables which organize a widely dispersed
literature. Boxes and annexes throughout the book explain technical
aspects, and twelve detailed experimental protocols, ranging from
in vitro folding of membrane proteins to single-particle electron
cryomicroscopy, have been contributed by and commented on by
experienced users. Membrane Proteins in Aqueous Solutions offers a
concise, accessible introduction to membrane protein biochemistry
and biophysics, as well as comprehensive coverage of the properties
and uses of conventional and non-conventional surfactants. It will
be useful both in basic and applied research laboratories and as a
teaching aid for students, instructors, researchers, and
professionals within the field.
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