The Advanced Methods series is intended for advanced
undergraduates, graduate students and established research
scientists. Titles in the series are designed to cover current
important areas of research in life sciences, and include both
theoretical background and detailed protocols. The aim is to give
researchers sufficient theory, supported by references, to take the
given protocols and adapt them to their particular experimental
systems. Since the first successful isolation and cultivation of
human embryonic stem cells at the University of Wisconsin - Madison
in 1998, there have been high levels of both interest and
controversy in this area of research. This book provides a concise
overview of an exciting field, covering the characteristics of both
human embryonic stem cells and pluripotent stem cells from other
human cell lineages. The following chapters describe
state-of-the-art differentiation and characterization of specific
ectoderm, mesoderm and endoderm-derived lineages from human
embryonic stem cells, emphasizing how these can be used to study
human developmental mechanisms. A further chapter discusses genetic
manipulation of human ES cells. The concluding section covers
therapeutic applications of human ES cells, as well as addresses
the ethical and legal issues that this research has raised.
General
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