Vincristine and vinblastine are two important anticancerous
alkaloids present in Catharanthus roseus. Isolation of these
compounds from in vivo grown plants is impeded by low productivity,
is therefore very expensive. Biotechnological methodologies have
recently been employed in improving the yield. In this monograph,
somatic embryogenesis - an important biotechnology based technique
has been described as to enrich alkaloid levels in C. roseus.
Biomass production by optimizing embryo proliferation, maturation
and large scale plantlets development has been discussed. The
influence of cultural conditions and other controlling factor's
role has been evaluated. The monograph discusses the applications
of overproducing mutant cell lines after successful in vitro
mutagenesis. The augmentation possibility of cell biomass by
employing large scale fermenter/ bioreactor has also been suggested
for commercial applications. The established protocol may offer an
ideal opportunity in developing Catharanthus transgenics enmasse.
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