This volume details the phenotypic characterization of
Staphylococcus aureus, with a focus on in vitro and ex vivo
methodologies. The chapters in this book cover topics such as in
vitro assessment of classical S. aureus virulence attributes;
quantifying promoter activity using a S. aureus codon-optimized
lacZ plasmid; biologically-relevant growth environments; metabolic
and stress resistance assays; and in vivo and ex vivo models of
host-pathogen interaction. Written in the highly successful Methods
in Molecular Biology series format, chapters include introductions
to their respective topics, lists of the necessary materials and
reagents, step-by-step, readily reproducible laboratory protocols,
and tips on troubleshooting and avoiding known pitfalls.
Cutting-edge and authoritative, Staphylococcus aureus: Methods and
Protocols is a valuable resource for anyone interested in this
fascinating and developing field.
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