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Studies on seed borne nature of Alternaria helianthi in Sunflower (Paperback)
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Studies on seed borne nature of Alternaria helianthi in Sunflower (Paperback)
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Sunflower is an important edible oilseed crop in India. The major
constraint in profitable sunflower cultivation is the
susceptibility to Alternaria leaf spot / blight caused by A.
helianthi. This has necessitated to study the implications of seed
borne nature of A. helianthi of sunflower on seed quality and to
find out suitable method for detection of A. helianthi. Studies on
cultural, morphological and molecular variation of A. helianthi
isolates collected from different locations in Andhra Pradesh were
also made. Significant adverse effects of A. helianthi on seed
quality parameters viz., germination, seed vigour and moisture
content and oil content were also observed. Among different
detection methods, Sunflower leaf extract medium (SLEM) was found
best in detection of the pathogen in the infected seed samples.
Molecular variability of A. helianthi isolates using RAPD analysis
with 43 random primers indicated distinct RAPD bands in OPM 16,
OPC11 and OPBC 2 markers. Irrespective of districts and genotypes,
isolates collected from sunflower seed samples does not exhibit any
correlation between cultural, morphological and molecular
characters studied.
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