Basic Methods for the Biochemical Lab elucidates proven lab
procedures and practical hints for research in analytical and
preparative biochemistry, as well as summarizing key data in
numerous tables. To further emphasize the practical aspects and to
minimize the text, theoretical introductions into the methods are
mostly omitted.
The chapters cover: quantitative methods (proteins, nucleic
acids, phospholipids and carbohydrates), electrophoresis (several
polyacrylamide and agarose systems, 2D-PAGE, detection methods and
affinity electrophoresis), chromatographic protocols (thin-layer
chromatography, GPC, IEC, affinity chromatography, HPLC),
immunochemical protocols (hapten-carrier and enzyme conjugation,
immunization and antibody purification, immune affinity
chromatography, ELISA), centrifugation (differential and density
gradient centrifugation for cells and cellular fractions),
radioactivity (labeling and counting), buffers (buffer properties
and compositions).
In additional chapters, tables compile a broad array of useful
information, e.g. SI units conversion factor, detergent, protein
and nucleotide data, and the basic principles of statistics and
enzyme and receptor kinetics are reviewed.
This first English-language edition of a successful
German-language manual will be a valuable resource for students and
experts in biochemistry, biotechnology and biomedical
laboratories.
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