Use of microtuber is advantageous than microplants but dormancy
problem of microtuber limits its production and use in the
laboratory and the field respectively. This book provides a proven
solution of dormancy breaking of freshly harvested microtuber with
5 to 7 days. A series of experiments in relation to production and
breaking of dormancy of potato microtubers were conducted during
2000-2003. Nitrogen at 45 and 60 mM with 300-500 mg/l CCC or 30-50
mg/l coumarin or 5 mg/l BAP or 10% sucrose produced higher
proportion of large size (>200 mg) microtubers after 105 days.
Treatment of intact microtuber with GA3 (0, 5, 10 and 15 mg/l) did
not show any noticeable effect on dormancy breaking, while soaking
of peeled microtubers treated with 0.1-1.0 mg/l GA3, 0.1 to 1.0
mg/l IAA and 0.01 to 0.1 mg/l Kin for 1 to 25 min and put on water
soaked cotton pad containing in petriplates showed >90%
sprouting within 7 days. Suberisation of peeled microtubers after
treatment had no effect on sprouting but was safe from rot, while
no rot of microtuber was observed while handling aseptically. This
book would be useful for the tissue culture laboratory as well as
basic seed potato growers.
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