Research into gluten sensitivity has never been more popular nor
more exciting. Thus a call for a new book, Celiac Disease: Methods
and Protocols, devoted entirely to techniques and technology seemed
a most appropriate undertaking. I am therefore grateful to
Professor J. M. Walker for inviting me to complete this task for
Humana Press. To do this would have been imp- sible without the
contributions of friends and colleagues from around the world who
have devoted so much interest to the project. It has also been
necessary for them to master the unique chapter-writing skills
required of every ma- script published in this series of laboratory
monographs. With regard to gluten sensitivity we are in a period of
great change, occasioned by the introduction of reproducible
methods for cloning lymp- cytes, the application of physical
methods to identify gluten sequences as T-cell antigens, the study
of peptide responses in vitro and in vivo by either jejunal or
rectal challenge, elucidating the locations of other genes
concerned in pathogenesis, or the use of elegant
immunohistocytochemical and mRNA probing techniques for analyzing
the finer points of the mucosal inflam- tory response to gluten.
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