Dr Spencer's account gives, in largely non-mathematical terms, a
concise but rigorous explanation of the principles underlying the
various forms of light microscopy. It starts with the properties of
lenses, elementary diffraction theory and theoretical limits to
resolution; it goes on to explain the workings of phase contrast,
dark-field, fluorescence, polarizing and interference microscopes.
There are chapters on aberrations and photomicrography, and
detailed instructions for the alignment and general care of
microscopes. Questions and answers are given at the end of each
chapter, and suggestions made for class experiments. Although
written primarily for undergraduates, the treatment is equally
suitable for those preparing for professional examinations in
microscopy, and for research workers seeking a better understanding
of the microscopes they use.
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