Advances in our understanding of biological mechanisms have
frequently been associated with the development of techniques. In
situ hybridisation is a classic case of just such an advance. The
technique effectively combines histochemistry with molecular
biology and enables the rapid analysis of the distribution of RNA,
or DNA, in the tissues. The information gained from this has caused
something of a revolution in our understanding of developmental
biology, since a fundamental aspect of development is the spatial
and temporal expression of genes. In addition the technique has
found application in the field of medicine, providing insights into
the functioning of healthy tissues and the diagnosis and study of
diseases. This book brings together contributions from leaders in
the application of in situ hybridisation and guides the would-be
exponent through the various options and variations of the
technique.
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