Advances in our understanding of biological mechanisms have
frequently been associated with the development of new techniques.
In situ hybridization is a classic case of just such an advance.
The technique effectively combines histochemistry with molecular
biology and enables the rapid analysis of the distribution of RNA,
or DNA, in the tissues. The information gained from this has caused
something of a revolution in our understanding of developmental
biology, since a fundamental aspect of development is the spatial
and temporal expression of genes. In addition the technique has
found application in the field of medicine, where it is giving new
insights into the functioning of healthy tissues and the diagnosis
and study of diseases. This book brings together contributions from
leaders in the application of this technique and will help the
reader review and be guided through the various options and
variations of the technique.
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