Single cell analysis has typically been difficult and challenging
to perform in such a way that meaningful statistical information
can be extracted. This thesis documents the development of a
microchip or lab-on-a-chip platform to perform single cell
electrophoresis in parallel separation channels. It describes the
use of optical tweezers to select a cell and transport it to a
desired separation channel. It also describes the use of
electromechanical shearing to lyse a cell. These two techniques are
combined with laser-micromachined separation channels to perform
simple electrohoresis on the fluorescently labeled cellular
contents.
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