A number of abiotic factors such as drought, salinity, extreme
temperatures, low or high light intensity, and deficiency or toxic
levels of nutrients have huge impacts on crop productivity, and a
furthering of our understanding of the molecular, biochemical, and
physiological basis of stress tolerance has been widely recognized
as critical. In Plant Stress Tolerance: Methods and Protocols,
expert researchers cover the most important widely-used techniques,
including cutting-edge strategies, in a manner that ensures
effective results. Beginning with reviews on dehydration, salinity,
and cold tolerance as well as on oxidative stress, the volume then
continues with methods involving topics such as describing the
identification of stress-regulated genes, proteins, and microRNAs
using diverse approaches, measurement of osmotic adjustment,
proline levels, enzymes involved in proline metabolism, and sugars
as well as determination of ROS levels, lipid peroxidation, ion
leakage, and the enzymes involved in ROS detoxification. Written in
the highly successful Methods in Molecular Biology (TM) series
format, chapters include introductions to their respective
subjects, lists of the necessary materials and reagents,
step-by-step, readily reproducible laboratory protocols, and tips
on troubleshooting and avoiding known pitfalls. Comprehensive and
up-to-date, Plant Stress Tolerance: Methods and Protocols provides
a wide range of easy-to-follow protocols catering to the needs of
plant physiologists, biochemists, and molecular biologists
interested in probing this vital area of study.
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