Biochemical methods are used in all branches of biological science including agriculture. Biochemical aspect is an integral part of plant physiology and this aspect is used to explain nearly all the phenomenon of physiological aspect of plant and/or crop. Technology and Methods for Biochemical Aspects of Plant Physiology is mainly intended for Post Graduate students and Researchers of Universities and of different Research Institutes. As it covers a broad range of subjects on the basic as well as the practical aspects of biochemical part of Plant Physiology, it is likely that it will be also useful for any student attending different theoretical or practical Plant Physiology as well as Biochemistry courses

Fruit Breeding is an art and science demanding prolonged time, space and intrinsic qualities. Seed propagation leads to heterogeneity and asexual propagation of elite clones' leads to newer and novel types. There are temperate, sub-tropical and tropical fruits differing in photo and thermo periodic requirements. There are also parthenocarpic and apomictic fruit trees demanding special breeding methods for improvement. Aroma, taste and flavor are unique to fruits unlike other horticultural crops like vegetables, spices, plantation crops and tubers. The book unravels history of fruit breeding, biodiversity, challenges approaches and prospects, apomixes, mutation breeding, role of insects in fruit production, molecular markers, statistical techniques, rootstock breeding, breeding of mango, citrus, banana, papaya, guava, sapota, jackfruit, sugarapple/sweetsop Indian jujube and jamun. The 20 s in the book authored by 15 scientists from six Research Institutes and Universities expose readers to a new world of fruit breeding, very seldom ventured by breeders.

Cashew (Anacardium occidentale L. f. Anacardiaceae) is very important and dollar earning plantation crop. A systematic compilation on various aspects has been covered in this book. It consists the s viz. Introduction; History, origin and geographical distribution; Area, production, productivity, export and import; Soil and climate; Biodiversity and crop improvement; Varieties; Propagation and production of planting material; Morphology and tree characters; Pollination techniques; Establishments of cashew orchard; Mineral nutrition; Training and pruning; Rejuvenation of old orchards; Inter-cropping and cover cropping; Intercultural operation; Maturity, harvesting and yield; Post harvest storage; Marketing of raw nuts; Nutritive value of cashew; Cashew nut processing; Cashew apple processing; Pests and diseases; Economics for cashew cultivation; Future strategies along with references. This book will be helpful to students and teachers of horticulture, agriculture and forestry, scientists, researchers, cashew growers and processors including small scale processing unit holders.

Techniques related to various physiological phenomenon are subject of tremendous interest and importance to plant physiologist, agronomist, horticulturist, ecologist, and biochemists. This book is intended to provide recognized methods related various plant processes in a comprehensive form. Techniques on crop physiology such as hydroponics and plant nutrition, test for various stresses, water potential and water flow in plants, canopy gas measurements (Photosynthesis, Respiration and Transpiration), basic equations for growth studies and methods for estimations of plant products, microclimate. Efforts were also made to incorporate the topic like Climate Change and theory of phytotron as well as rhizotron in this book. The book will make the reader familiar with latest procedure to elucidate the problems. The validity of the results based on fundamentals principles of physics. This book is meant to be used in conjunction with a standard text of plant physiology though elementary principles relating to the techniques are briefed. The subjects on hormones, tissue culture and seed technology are useful for students. Hope this book shall serve the need of students, teachers and researchers.

The book covers information on various types of mutagens and their effects, procedures for using mutagens for crop improvement, types of mutations (micro and macro) with statistical techniques to handle the mutation population. The subject matter presented in the book will be useful for both undergraduate and post graduate students of agriculture.

Floriculture is one of the fastest-growing sectors of commercial agriculture world-wide with many highly profitable crops. Cultivation of flowers more pragmatic endeavor than other crops. Every day novel variety, new colour in any ornamental flower crop not only fascinates us but also gives a thrust to know more about their breeding technologies. Creation of diversity of new and domesticated flower crops by public and private sector flower breeders brings a fascination towards its breeding technology. The students while dealing with the breeding and biotechnology of flowers then they must required a base knowledge. Therefore emphasis has been given to present the book in its easiest form so that anyone can understand it without losing interest from it. It has been designed to cover all the aspects of breeding, the basic objectives, different breeding methods, methodology for improvement of specific crops, stress resistance, quality improvement, mutagenesis, genetic engineering and biotechnology.

Flowers are the precious gift which beautify the nature through its different colours and enhance human health. Ornamental plants provide environmental security for immediate living surroundings, thus intensive research in floriculture particularly on the crops were initiated earlier. This book will vividly highlights genetical and breeding application in flower crops covering wide range of aspects. Breeding techniques are largely focused around expediting the production of superior and stable lines in the case of self pollinating crops. Wide hybridization, tissue culture and mutagenesis are employed by breeders to generate new alleles. Broaden available genetic resources; molecular markers are used to assist breeders through marker assisted selection and to identify quantitative trait loci for traits of interest. The book makes the knowhow of breeding in its easiest way to the readers. It has been designed to cover all the aspects of breeding, the basic objectives, different breeding methods, methodology for improvement of specific crops, stress resistance, quality improvement, mutagenesis, molecular breeding and genetic engineering.

In modern days, crop improvement is a multidisciplinary division of agriculture. In this book, entitled, "Breeding, Biotechnology and Seed Production of Field Crops", emphasis has been given on principles, methods and practices in plant breeding, biotechnology in crop improvement and seed production of field crops. The book has been written for all sections of learners, educators and staff-members of seed industries. Particular importance has been underlined for postgraduate students who specialize in plant breeding and seed science. Each of the book has been designed as per the recommended of syllabus of Indian Council of Agricultural Research for the postgraduate students of various Agricultural Universities in our country. This book has been divided into two major parts- i) Principles of crop breeding and ii) Methods and practices of crop improvement and seed production of individual field crop. The book contains total of 18 chapteFirst three s are related to shed light on the basic-principles and remaining s deal with methods and practices of individual crop for improvement and seed production. We hope that the book will be ready to lend a hand to the advanced undergraduate students doing plant breeding in elective, postgraduate students who opted plant breeding, teachers, researchers and staff-members of private seed companies of this field of specialization.

The latest and most commonly used methods of assay of important enzymes associated with carbon, nitrogen, protein and lipid metabolism. Estimation of various plant pigments and micro and macro elements. Quantification of plant hormones like IAA, ABA, GA and Ethylene. Techniques of DNA and RNA estimation, Slab Gel Electrophoresis and Western Blot analysis of plant proteins. Methods to study plant biomass and plant-water relationship. Methods to measure photosynthesis and respiration. Method for preparation of common buffe Working principles and operation techniques of a few analytical equipments like Infra-Red Gas Analyzer IRGA, Gas Liquid Chromatograph GLC, Psychrometer, Pressure bomb/pressure chamber, flame photometer, atomic absorption spectrophotometer, Leaf Area Meter and Oxygen electrode. This book is useful for students in botany, plant physiology, biochemistry, horticulture, agronomy and other cognate disciplines and other research workers."

Abiotic stress has a detrimental impact on the living organisms in a specific environment and constitutes a major constraint to global agricultural production. The adverse environmental conditions that plants encounter during their life cycle not only disturb their metabolic reactions, but also hamper their growth and development on cellular and whole plant levels. These conditions are of great concern, particularly for those countries whose economies primarily rely on agriculture. Under abiotic stresses, plants amalgamate multiple external stress cues to bring about a coordinated response and establish mechanisms to mitigate such stresses by triggering a cascade of events leading to enhanced tolerance.

"Physiological Mechanisms and Adaptation Strategies in Plants under Changing Environment," "Volume 2" displays the ways by which plants utilize and integrate many common signals and subsequent pathways to cope with less favourable environmental conditions." "The book also describes the use of contemporary tools for the improvement of plants under such stressed environments. Concise yet comprehensive, "Physiological Mechanisms and Adaptation Strategies in Plants under Changing Environment," "Volume 2" is an indispensable resource for researchers, students, environmentalists and many others in this burgeoning area of research."

Abiotic and biotic stresses adversely affect plant growth and productivity. The phytohormones regulate key physiological events under normal and stressful conditions for plant development. Accumulative research efforts have discovered important roles of phytohormones and their interactions in regulation of plant adaptation to numerous stressors. Intensive molecular studies have elucidated various plant hormonal pathways; each of which consist of many signaling components that link a specific hormone perception to the regulation of downstream genes. Signal transduction pathways of auxin, abscisic acid, cytokinins, gibberellins and ethylene have been thoroughly investigated. More recently, emerging signaling pathways of brassinosteroids, jasmonates, salicylic acid and strigolactones offer an exciting gateway for understanding their multiple roles in plant physiological processes. At the molecular level, phytohormonal crosstalks can be antagonistic or synergistic or additive in actions. Additionally, the signal transduction component(s) of one hormonal pathway may interplay with the signaling component(s) of other hormonal pathway(s). Together these and other research findings have revolutionized the concept of phytohormonal studies in plants. Importantly, genetic engineering now enables plant biologists to manipulate the signaling pathways of plant hormones for development of crop varieties with improved yield and stress tolerance. This book, written by internationally recognized scholars from various countries, represents the state-of-the-art understanding of plant hormones' biology, signal transduction and implications. Aimed at a wide range of readers, including researchers, students, teachers and many others who have interests in this flourishing research field, every section is concluded with biotechnological strategies to modulate hormone contents or signal transduction pathways and crosstalk that enable us to develop crops in a sustainable manner. Given the important physiological implications of plant hormones in stressful environments, our book is finalized with chapters on phytohormonal crosstalks under abiotic and biotic stresses.

The book is exceptional in its organization with three major characteristics of plant system i.e. Plant Physiology, Biochemistry and Molecular Biology been provided under one canopy. Physiology, which deals with all the vital activities of a plant and also explains how it reacts to sustain in natural distress similarly within the plant, the types of physiological actions at biochemical level forming innumerable compounds through chains of biochemical reactions at various levels of plant growth and development becomes Biochemistry. However, the curiosity and thirst of knowledge of human being is endless. Man has been providing still inside up to the molecular and genetic levels to understand the nature of biochemical reactions and to control if possible up to the desired level and that is Molecular Biology. Now this is the time to elevate most relevant work of academic and applied importance out of vast research of diverse significance done in the last fifty years.

This textbook is clearly structured with fourteen richly illustrated chapters and practical examples for easy understanding and direct implementation. The methods and findings developed in the authors' group are presented in detailed, revised chapters. Readers will find valuable updates on the molecular basis of biotechnological processes, secondary metabolite production and genetic engineering. In addition, the basic principles of important biotechnologies, as well as examples of specially designed crops that deliver improved productivity under stress conditions, are presented. This second edition sets the direction for future research on the basic aspects of plant tissue culture and its applications in the fields of secondary metabolite production and genetic engineering. It provides both general and specific information for students, teachers, academic researchers and industrial teams who are interested in new developments in plant tissue culture and its applications.

This book focuses on the effects of genotoxic agents causing oxidative stress in plants. The book explores different kind of chemicals which induces genotoxicity, their mechanism of action and effects on plant health. Impacts at the physiological and molecular levels are discussed. The book is of interest to teachers, researchers and plant scientists. Also the book serves as additional reading material for undergraduate and graduate students of agriculture, forestry, ecology, soil science, and environmental sciences. National and international agricultural scientists will also find this to be a useful read.

Medicinal plants are globally valuable sources of herbal products. Plant-based remedies have been used for centuries and have had no alternative in the western medicine repertoire, while others and their bioactive derivatives are in high demand and have been the central focus of biomedical research. As Medicinal plants move from fringe to mainstream with a greater number of individuals seeking treatments free of side effects, considerable attention has been paid to utilize plant-based products for the prevention and cure of human diseases. An unintended consequence of this increased demand, however, is that the existence of many medicinal plants is now threatened, due to their small population size, narrow distribution area, habitat specificity, and destructive mode of harvesting. In addition, climate change, habitat loss and genetic drift have further endangered these unique species. Although extensive research has been carried out on medicinal and aromatic plants, there is relatively little information available on their global distribution patterns, conservation and the associated laws prevailing. This book reviews the current status of threatened medicinal plants in light of increased surge in the demand for herbal medicine. It brings together chapters on both wild (non-cultivated) and domestic (cultivated) species having therapeutic values. Thematically, conventional and contemporary approaches to conservation of such threatened medicinal plants with commercial feasibility are presented. The topics of interest include, but not limited to, biotechnology, sustainable development, in situ and ex situ conservation, and even the relevance of IPR on threatened medicinal plants. We believe this book is useful to horticulturists, botanists, policy makers, conservationists, NGOs and researchers in the academia and the industry sectors.

This book summarizes the latest studies on plant reproduction and multiple aspects of signaling in reproductive development. It also presents the most advanced processes in CrRLK1L receptor and RALF peptide studies during plant development. Focusing on signaling in pollen tube integrity and sperm release regulation, it provides significant insights into the BUPS-ANX receptor complex and the corresponding ligands RALF4/19 to promote pollen tube growth with proper cell integrity. It also proposes a working model of female tissue-derived RALF34 competing with RALF4/19 from the BUPS-ANX to trigger pollen tube rupture and sperm release. Offering a detailed overview of the spatiotemporal regulation mechanism underlying the control of pollen tube integrity and sperm release, the book fills a major gap in our understanding of plant reproductive processes, and as such is a valuable resource for those working in the area of plant signaling.

This book presents recent developments involving the role of nanoparticles on plant physiology and growth. Nanotechnology applications include improvement of agricultural production using bio-conjugated NPs (encapsulation), transfer of DNA in plants for development of insect pest-resistant varieties, nanoformulations of agrochemicals such as pesticides and fertilizers for crop improvement, and nanosensors/nanobiosensors in crop protection for identification of diseases and residues of agrochemicals. Recent findings on the increased use of nanotechnology in agriculture by densely populated countries such as China and India indicate that this technology may impart a substantial impact on reducing hunger, malnutrition, and child mortality.

Refinement in sequencing technologies and potential of genomic research resulted in meteoric growth of biological information such as sequences of DNA, RNA and protein requiring databases for efficient storage, management and retrieval of the biological information. Also, computational algorithms for analysis of these colossal data became a vital aspect of biological sciences. The work aims to show the process of turning bioscience innovation into companies and products, covering the basic science, the translation of science into technology. Due to rapid developments, there seems to be no basic difference between the pharmaceutical industry and the biotechnological industry. However, approved products in the pipeline and renewed public confidence make it one of the most promising areas of economic growth in the near future. India offers a huge market for the products as well as cheap manufacturing base for export. The book is a sincere work of compilation of new and recent advances in the topic of concern through various innovative researches and scientific opinion therefrom. The book is dedicated to the readers who will definitely find it interesting and knowledgeable in carrying out their respective researches in different aspects of applied microbiology and biotechnology.