Leaf longevity is a fundamental process underlying patterns of variation in foliar phenology and determining the distinction between deciduous and evergreen plant species. Variation in leaf longevity is associated with a wide array of differences in the physiology, anatomy, morphology and ecology of plants. This book brings together for the first time information scattered widely in the botanical literature to provide a clear and comprehensive introduction to the nature and significance of variation in leaf longevity. It traces the development of ideas about leaf longevity from the earliest descriptive studies to contemporary theory of leaf longevity as a key element in the function of leaves as photosynthetic organs. An understanding of variation in leaf longevity reveals much about the nature of adaptation at the whole plant level and provides fundamental insights into the basis of variation in plant productivity at the ecosystem level. The analysis of leaf longevity also provides a process-based perspective on phenological shifts associated with the changing climate. Readers will find this an informative synthesis summarizing and illustrating different views in a readily accessible narrative that draws attention to a central but too often unappreciated aspect of plant biology. The nature and causes of seasonal patterns in the birth and death of individual plant leaves are essential to the understanding of the health of plant communities, biomes, and consequently our planet.

With one volume each year, this series keeps scientists and advanced students informed of the latest developments and results in all areas of the plant sciences. This latest volume includes reviews on plant physiology, biochemistry, genetics and genomics, forests, and ecosystems.

Plant protoplasts have proved to be an excellent tool for in vitro manipu- lations, somatic hybridization, DNA uptake and genetic transformation, and for the induction of somaclonal variation. These studies reflect the far- reaching impact of protoplast alterations for agriculture and forest bio- technology. Taking these aspects into consideration, the series of books on Plant Protoplasts and Genetic Engineering provides a survey of the litera- ture, focusing on recent information and the state of the art in protoplast Plant Protoplasts manipulation and genetic transformation. This book, and Genetic Engineering VI, like the previous five volumes published in 1989,1993, and 1994, is unique in its approach. It comprises 27 chapters dealing with the regeneration of plants from protoplasts, and genetic transformation in various species of Arachis, Bupleurum, Capsella, Dendrobium, Dianthus, Diospyros, Fagopyrum, Festuca, Gentiana, Glycyrrhiza, Gossypium, Hemerocallis, Levisticum, Lonicera, Musa, Physallis, Platanus, Prunus, Saposhnikovia, Solanum, Spinacia, Trititrigia, Tulipa, and Vaccinium; including fruits such as apricot, banana, cranberry, pepino, peach, and plum. This book may be of special interest to advanced students, teachers, and research scientists in the field of plant tissue culture, molecular biology, genetic engineering, plant breeding, and general bio- technology. New Delhi, August 1995 Professor Y. P. S. BAJA] Series Editor Contents Section I Regeneration of Plants from Protoplasts 1. 1 Regeneration of Plants from Protop1asts of Arachis Species (Peanut) Z. LI, R. L. JARRET, and J. W. DEMSKI (With 2 Figures) 1 Introduction ...3 2 Isolation of Pro top lasts ...4 3 Culture of Protoplasts ...

Abiotic stresses such as high temperature, low-temperature, drought, and salinity limit crop productivity worldwide. Understanding plant responses to these stresses is essential for rational engineering of crop plants. In Arabidopsis, the signal transduction pathways for abiotic stresses, light, several phytohormones and pathogenesis have been elucidated. A significant portion of plant genomes (most studies are Arabidopsis and rice genome) encodes for proteins involves in signaling such as receptor, sensors, kinases, phosphatases, transcription factors and transporters/channels. Despite decades of physiological and molecular effort, knowledge pertaining to how plants sense and transduce low and high temperature, low-water availability (drought), water-submergence and salinity signals is still a major question before plant biologists. One major constraint hampering our understanding of these signal transduction processes in plants has been the lack or slow pace of application of molecular genomic and genetics knowledge in the form of gene function. In the post-genomic era, one of the major challenges is investigation and understanding of multiple genes and gene families regulating a particular physiological and developmental aspect of plant life cycle. One of the important physiological processes is regulation of stress response, which leads to adaptation or adjustment in response to adverse stimuli. With the holistic understanding of the signaling pathways involving not only one gene family but multiple genes or gene families, plant biologists can lay a foundation for designing and generating future crops that can withstand the higher degree of environmental stresses (especially abiotic stresses, which are the major cause of crop loss throughout the world) without losing crop yield and productivity.

The study of water stress is one of the most interesting subjects in. the investigation of water relations in plants. From the theoretical point of view it is concerned with investigating the mechanisms of the distribution and movement of water in the plant organism and the way in which physiolo gical processes are influenced by water deficiency. From the practical point of view, water deficiency is a major factor limiting plant production. It has been progressively shown that water deficiency is not by far* only a factor in plant life in dry climates, that obvious wilting is not the first warning sign of water deficiency and that moderate water stress, caused by temporary negative water balance during the day, affects physiological ac tivity and decreases prodnction in the ecological conditions of the temperate zone. In addition, even general water deficiency is not today confined to arid or semi-arid zones and to the absolutely dry season of the year. The tremend ous consumption of water in our civilization has become today, even in the temperate zone, an important competitor with the plant cover. The study of water relations from the aspect of water stress is, therefore, important both theoretically and practically. I assume, therefore, that it was useful, important and interesting to meet in a symposium on water stress in plants and to discuss, as far as possible, in detail problems which are obviously among the main, whose solution would help plant physiology in increasing and improving plant production.

The analysis of changes in gene activity in tissues and cells of plants is an important way of measuring developmental and environmental responses. This is achieved by identifying, isolating, and analyzing the genes responsible for these changes, and assessing their degree of genetic expression in relation to other cells and tissues within each plant. This book, designed as a manual, provides detailed accounts of new and established techniques used to carry out these analyses, as well as a section on trouble-shooting.

This text details the plant-assisted remediation method, "phytoremediation," which involves the interaction of plant roots and associated rhizospheric microorganisms for the remediation of soil contaminated with high levels of metals, pesticides, solvents, radionuclides, explosives, crude oil, organic compounds and various other contaminants. Each chapter highlights and compares the beneficial and economical alternatives of phytoremediation to currently practiced soil removal and burial practices.

The Genetics and Genomics of the Brassicaceae provides a review of this important family (commonly termed the mustard family, or Cruciferae). The family contains several cultivated species, including radish, rocket, watercress, wasabi and horseradish, in addition to the vegetable and oil crops of the Brassica genus. There are numerous further species with great potential for exploitation in 21st century agriculture, particularly as sources of bioactive chemicals. These opportunities are reviewed, in the context of the Brassicaceae in agriculture. More detailed descriptions are provided of the genetics of the cultivated Brassica crops, including both the species producing most of the brassica vegetable crops (B. rapa and B. oleracea) and the principal species producing oilseed crops (B. napus and B. juncea). The Brassicaceae also include important "model" plant species. Most prominent is Arabidopsis thaliana, the first plant species to have its genome sequenced. Natural genetic variation is reviewed for A. thaliana, as are the genetics of the closely related A. lyrata and of the genus Capsella. Self incompatibility is widespread in the Brassicaceae, and this subject is reviewed. Interest arising from both the commercial value of crop species of the Brassicaceae and the importance of Arabidopsis thaliana as a model species, has led to the development of numerous resources to support research. These are reviewed, including germplasm and genomic library resources, and resources for reverse genetics, metabolomics, bioinformatics and transformation. Molecular studies of the genomes of species of the Brassicaceae revealed extensive genome duplication, indicative of multiple polyploidy events during evolution. In some species, such as Brassica napus, there is evidence of multiple rounds of polyploidy during its relatively recent evolution, thus the Brassicaceae represent an excellent model system for the study of the impacts of polyploidy and the subsequent process of diploidisation, whereby the genome stabilises. Sequence-level characterization of the genomes of Arabidopsis thaliana and Brassica rapa are presented, along with summaries of comparative studies conducted at both linkage map and sequence level, and analysis of the structural and functional evolution of resynthesised polyploids, along with a description of the phylogeny and karyotype evolution of the Brassicaceae. Finally, some perspectives of the editors are presented. These focus upon the Brassicaceae species as models for studying genome evolution following polyploidy, the impact of advances in genome sequencing technology, prospects for future transcriptome analysis and upcoming model systems.

This symposium on 'Boron in Soils and Plants' completes a quartet of reviews of the behaviour in soils and plants of four trace elements, copper, manganese, zinc and boron, selected for their importance in agriculture. The series had its genesis in a suggestion from Professor Robin Graham of the Waite Agricultural Research Institute that it would be appropriate in 1981 to celebrate the Golden Jubilee of the publication in 1931 of the first definitive evidence for the essentiality of copper in plants. The previous decade had seen a resurgence of interest in copper deficiency and toxicity in agriculture and an expansion of our understanding of the behaviour of copper in soils and plants. We therefore decided to promote a review of our understanding of the behaviour of copper in soils and plants by inviting appropriate authors to prepare reviews of 14 topics for publication in a book and presentation at a Golden Jubilee International Symposium on 'Copper in Soils and Plants'. Posters of current research were also displayed and published. Murdoch University, Perth, Western Australia was chosen as the venue because of its then current research on copper, the importance of copper in Western Australian agriculture, and the presence in Perth of many international nutritionists due to the fortuitous scheduling in 1981 of the 'Fourth International Symposium on Trace Element Metabolism in Man and Animals'.

The symposium on "Zinc in Soils and Plants" is the third in a series which began with "Copper in Soils and Plants" in Perth in 1981 and continued with "Manganese in Soils and Plants" in Adelaide in 1988. The symP9sium brings together a series of valuable accounts of many aspects of the reactions of zinc in soils, the uptake, transport and utilization of zinc in plants, the diagnosis and correction of zinc deficiency in plants and the role of zinc in animal and human nutrition. I am grateful for the financial support provided by Grains Research and Development Corporation, Rural Industries Research and Development Corporation, Wool Research and Development Corporation, Ansett Australia, and Qantas Australian. I am most appreciative of the willingness of many scientists to act as referees: G S P Ritchie, R J Gilkes, N C Uren, K Tiller, BLeach, H Greenway, N E Longnecker, J F Loneragan, Z Rengel, C A Atkins, J W Gartrell, P J Randall, D G Edwards, R J Hannam, R J Moir, J E Dreosti, N Suttle, C L White, H Marschner, N Wilhelm, M McBride. All provided valuable comments on the manuscripts. Finally, I thank Mrs M Davison who provided excellent secretarial assistance. A.D. Robson September 1993 Chapter 1.

Primary Active Transporters: A Plethora of Plant Plasmalemma Proton Pumps (M.R. Sussman). Studies on the Higher Plant CalmodulinStimulated ATPase (D.E. Evans et al.). Secondary Ion and Metabolite Transporters: ProtonSugar Cotransporters in Plants (N. Sauer). Insights into the Structure of the Chloroplast Phosphate Translocator Protein (H. Wallmeier et al.). Channel Proteins: Soybean Nodulin26 (D.P.S. Verma). Putative LType Calcium Channels in Plants (R. Ranjeva et al.). Receptor Proteins: Hormone Perception and Signal Transduction in Aleurone (R. Hooley et al.). The Auxin Receptor (R.M. Napier, M.A. Venis). Protein Targeting and Assembly in Membranes: Sequence Determinants for Protein Import into Chloroplasts and Thylakoid Membrane Protein Assembly (G. von Heijne). 11 additional articles. Index.

Global industrial growth has resulted in numerous pollutants being introduced into the environment. It has additionally caused decreased water availability for agricultural activity in developing countries, which, in turn, has compelled farmers to use wastewater irrigation. In advanced agricultural systems, farmers are adapting various strategies to achieve a higher yield and thus sustain crop productivity. Consequent to the introduction of contaminants in the environment, soil pollutants have become a critical issue. Selection of disease-resistant, high-yielding crop varieties, and extensive fertilizer applications are quite common among farming communities. This book provides insight into environmental pollutants with special reference to their interference with plant nutrition. It additionally discusses the physiological aspects of plant nutrition. This book enhances current knowledge of the effects of pollutants on plant growth and physiology.

Finally - a guide to cytological techniques written specifically for the plant chromosome researcher and student. Plant Chromosomes: Laboratory Methods thoroughly covers all important approaches to the study of plant chromosomes. It reviews each specific approach and describes requisite experimental techniques. These practical descriptions cover basic, standard techniques as well as the most recent research advances and state-of-the-art technologies.
Plant Chromosomes: Laboratory Methods allows you to build on the knowledge of its expert authors, who have first-hand experience with the ins and outs of each approach. Through hundreds of trouble-shooting suggestions it also helps you avoid experimental pitfalls by providing invaluable tips at critical points in the experimental process. This book gives you the information you need to improve the power of your plant chromosome research - saving you time and effort in the process. No other single volume contains so much practical information on this topic.

Most plants rely on the co-existence with microorganisms: both groups benefit from these symbioses. It has been shown that a large number of specific genes in plants and microorganisms are only activated during these interactions. Of course, various microbes also act as pathogens.

Interactions between plants and microorganisms are often located on plant surfaces, such as leaf cuticles, seeds and mainly on the roots. The communication between plants and microbes is the main topic treated in "Plant Surface Microbiology," such as the signaling within a symbiosis, the molecular differences between symbiotic and pathogenic microorganisms, the role of microorganisms in the development of plants or in plant protection against deleterious agents. Further contributions are devoted to: the analysis of bacterial communities in the rhizosphere; microbial population genetics; aspects of mycorrhizal symbiosis; functional genomic approaches and the use of microorganisms as bio-indicator of soil disturbance.

Adopting an interdisciplinary approach to the study of photoassimilate partitioning and source-sink relationhips, this work details the major aspects of source-sink physiology and metabolism, the integration of individual components and photoassimilate partitioning, and the whole plant source-sink relationships in 16 agriculturally important crops. The work examines in detail the components of carbon partitioning, such as ecology, photosynthesis, loading, transport and anatomy, and discusses the impact of genetic, environmental and agrotechnical factors on the parts of whole plant source-link physiology.

The enormous amount of data now available about the pollen tube clearly reflects its qualities as a biological model that go much beyond that of a carrier of sperm cells essential for plant reproduction. The diversity of techniques and methodologies currently used to study pollen and pollen tube growth is reflected in this book written by biochemists, cell biologists, molecular biologists and geneticists. Their different perspectives demonstrate that pollen tubes are excellent models for plant cell research, particularly suitable for investigations on cell tip growth and polarization, signal transduction, channel and ion flux activity, gene expression, cytoskeleton and wall structure, membrane dynamics and even cell cell communication.

This book covers the important diseases and pests of potato which are of global significance. The pests and diseases in potato lead to huge economic losses by reducing the yield and quality of the produce. This book describes major pests and diseases in detail with particular emphasis on the latest developments with respect to their biology, ecology, and management. It highlights the importance of virus infection for seed potato production and diagnostic symptoms, along with management guidelines. The book brings forth tips for judicious use of pesticides for sustainable potato production and management of pesticide resistance. Use of novel approaches such as RNA interference, genome editing, and other genomic resources for drug designing in diseases and pest management is also emphasized in the book. This book is of interest to teachers, researchers, extension workers, potato growers, and policy makers. Also, the book serves as additional reading material for undergraduate and graduate students of agriculture and plant pathology. National and international agricultural scientists and policy makers will also find this to be a useful read.

The book is a fundamental reference source on reaction wood for wood scientists and technologists, plant biologists, silviculturists, forest ecologists, and anyone involved in the growing of trees and the processing of wood. It brings together our current understanding of all aspects of reaction wood, and is the first book to discuss both compression wood and tension wood.

Trees produce reaction wood to maintain the vertical orientation of their stems and the optimum angle of each branch. They achieve this by laying down fibre cell walls in which differences in physical and chemical structure from those of normal fibres are expressed as differential stresses across the stem or branch. This process, while of obvious value for the survival of the tree, causes serious problems for the utilisation of timber. Timber derived from trees containing significant amounts of reaction wood is subject to dimensional instability on drying, causing twisting, bending and splitting. It is also difficult to work as timber, and for the pulp and paper industry the cost of removing the increased amount of lignin in compression wood is substantial. This has both practical and economic consequences for industry.

Understanding the factors controlling reaction wood formation and its effect on wood structure is therefore fundamental to our understanding of the adaptation of trees to their environment and to the sustainable use of wood.

The topics covered include:

-Morphology, anatomy and ultrastructure of reaction wood

-Cell-wall polymers in reaction wood and their biosynthesis

-Changes in tree proteomes during reaction wood formation

-The biomechanical action and biological functions of reaction wood

-Physical and mechanical properties of reaction wood from the scale of cell walls to planks

-The detection and characterisation of compression wood

-Effects of reaction wood on the performance of wood and wood-based products

-Commercial implications of reaction wood and the influence of forest management on its formation

"

This collection features four peer-reviewed reviews on the nutritional and health benefits of beverage crops. The first chapter introduces the importance of coffee leaves, highlighting their high antioxidant potential and impact on fruit quality. The chapter provides an inventory of molecules identified in the leaves of cultivated coffee trees, as well as the beneficial effects of these molecules on human health. The second chapter considers the nutritional and health-related aspects of regular coffee consumption, focussing on its ability to prevent the onset of chronic diseases. The chapter also highlights that above-average consumption of coffee can lead to the development of side effects, including caffeine tolerance, dependence and withdrawal. The third chapter discusses the main phytochemicals contained in tea, including polyphenols, amino acids, vitamins, carbohydrates and purine alkaloids. The chapter reviews the current analytical techniques available for tea characterisation, such as chromatic and spectroscopic techniques. The final chapter explores the beneficial health effects of consuming tea on a regular basis. The chapter considers the potential role for tea in combatting chronic diseases, such as cancer, diabetes, cardiovascular and neurodegenerative diseases, as well as the possible mechanisms of actions of tea constituents.

The Plant Hormone Ethylene: Stress Acclimation and Agricultural Applications presents current knowledge on our understanding of ethylene perception and signaling, its role in the regulation of plant physiological processes, and its contribution to acclimation in stressful environments. Plants regularly face environmental constraints due to their immobile nature. In persistently changing environmental conditions, several stress factors influence cellular metabolism, ultimately causing reduced plant growth and development with a significant loss in agricultural productivity. Sustainable agriculture depends on the acclimation of plant processes to the changing environment through altered physiological and molecular responses, which are controlled by plant hormones, including ethylene. Ethylene interacts with other plant hormones and signaling molecules to regulate several cellular processes, plant growth and development, and, ultimately, crop productivity. This book begins with an introduction to ethylene before providing a detailed study of the latest findings on the role of ethylene in plants, including its role in photosynthetic processes, flower development, leaf senescence, nutrients acquisition, and regulation of abiotic stress responses as well as its application in agriculture. The book is an ideal guide for researchers exploring plant physiology and biochemistry as well as for those investigating the use of ethylene knowledge in agriculture in persistently changing environmental conditions.

The future of agriculture strongly depends on our ability to enhance productivity without sacrificing long-term production potential. An ecologically and economically sustainable strategy is the application of microorganisms, such as the diverse bacterial species of plant growth promoting bacteria (PGPB). The use of these bio-resources for the enhancement of crop productivity is gaining worldwide importance.

""Bacteria in Agrobiology: Plant Probiotics"" discusses the current trends and future prospects of beneficial microorganisms acting as Probiotics. Topics include the application for the aboveground fitness of plants, in mountain ecosystems, in tropical and Mediterranean forests, and in muga sericulture. Further aspects are "Arabidopsis" as a model system for the diversity and complexity of plant responses, plant parasitic nematodes, nitrogen fixation and phosphorus nutrition."

This book provides relevant findings on nanoparticles' toxicity, their uptake, translocation and mechanisms of interaction with plants at cellular and sub-cellular level. The small size and large specific surface area of nanoparticles endow them with high chemical reactivity and intrinsic toxicity. Such unique physicochemical properties draw global attention of scientists to study potential risks and adverse effects of nanoparticles in the environment. Their toxicity has pronounced effects and consequences for plants and ultimately the whole ecosystem. Plants growing in nanomaterials-polluted sites may exhibit altered metabolism, growth reduction, and lower biomass production. Nanoparticles can adhere to plant roots and exert physicochemical toxicity and subsequently cell death in plants. On the other hand, plants have developed various defense mechanisms against this induced toxicity. This books discusses recent findings as well as several unresolved issues and challenges regarding the interaction and biological effects of nanoparticles. Only detailed studies of these processes and mechanisms will allow researchers to understand the complex plant-nanomaterial interactions.

Sustainable Plant Nutrition: Molecular Interventions and Advancements for Crop Improvement explores the significant opportunities for sustainable, eco-friendly approaches in plant nutrition and agricultural crop production. The book highlights the various prospects involved in optimizing plant nutrient uptake agriculture and includes chapters representing diverse areas dealing with biotechnology, nanotechnology, molecular biology, proteomics, genomics and metabolomics. This book is an ideal resource for those seeking to ensure a sustainable plant production future. While plants have evolved a set of elaborate mechanisms to cope with nutrient limitations, the traditional supplementation by the application of fertilizers to plant productivity may then lead to overfertilization which can actually reduce plant growth and have adverse effects on the environment. To tackle these issues, a detailed understanding of the responses of plants to nutrients and nutrient deficiency at the physiological, metabolic, transcriptome and epigenetic level is essential.

Invasive species have inspired concern for many reasons, including economic and environmental impacts in specific jurisdictions within particular countries. However, it is apparent that for some invasive plant species, political borders offer only weak barriers because these species have succeeded in invading many countries, emerging as threats at a global level. With this level of threat, a number of books on invasive plants and invasive species in general have been published in recent years, but none explicitly provides "global" coverage, perhaps because it is only recently that the full geographical, economic and environmental implications of widespread spread and adaptive nature of these particular invasive plants have been recognized. We plan to make this volume unique by profiling plant invasions in explicitly geographical contexts; on the world continents (Chapters 5-11), as well as islands (Chapter 12) and mountains (Chapter 13). This global approach is supported by an overview of invasion biology and recent advances (Chapter 1) and how different communities differ in invasibility (Chapter 2). Global factors influencing invasion are introduced in Chapter 3 (globalized trade) and Chapter 4 (climate change). Key species are profiled through geographic treatments, continent by continent (Chapters 5-11), and for islands (Chapter 12) and mountains (Chapter 13). The impact of invasive plants is highlighted in Chapter 14, both in biotic and economic terms, partly to counter the tendency for the young field of invasion biology to rely too much on anecdotal evidence. This chapters is also designed to bring home the message that these are serious problems that must be dealt with, as covered in the subsequent chapters. The book concludes with three chapters casting light on solutions to the many problems described in the rest of the volume. Chapter 15 features new, innovative technologies that are being developed to monitor and manage invasive plants, and Chapter 16 presents comprehensive strategies for public education and implementation of management on local and global scales. Chapter 17 describes different future scenarios depending on current trends in plant invasion and its management, just as climate change predictions employ various scenarios to project the future. The future is very much up to us, as humanity grapples with the question of how best to strategically meet the problems of global invasive plant problems that we ourselves have created that is further challenged by a changing climate. We are confident that this book will be of interest to invasion biologists, resource managers, and the legion of others who must deal with these invasive plants across the globe on a daily basis.

This book examines the state-of-the-art on plants and fibres as building materials for low cost construction, emphasizing their use, properties, fabrication, new procedures and future developments. It makes available research results on new techniques for fibre reinforcement and their use in concrete, stabilized clay and other matrices. Procedures for making vegetable fibres and wood-based building materials in developing countries are also analysed.